Gene description for EFNB2
Gene name ephrin-B2
Gene symbol EFNB2
Other names/aliases EPLG5
HTKL
Htk-L
LERK5
Species Homo sapiens
 Database cross references - EFNB2
ExoCarta ExoCarta_1948
Vesiclepedia VP_1948
Entrez Gene 1948
HGNC 3227
MIM 600527
UniProt P52799  
 EFNB2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for EFNB2
Molecular Function
    virus receptor activity GO:0001618 IEA
    protein binding GO:0005515 IPI
    ephrin receptor binding GO:0046875 IBA
    ephrin receptor binding GO:0046875 IPI
Biological Process
    lymph vessel development GO:0001945 IEA
    cell migration involved in sprouting angiogenesis GO:0002042 IDA
    cell adhesion GO:0007155 IDA
    cell-cell signaling GO:0007267 TAS
    axon guidance GO:0007411 IBA
    positive regulation of cell population proliferation GO:0008284 IMP
    anatomical structure morphogenesis GO:0009653 TAS
    animal organ morphogenesis GO:0009887 IEA
    negative regulation of keratinocyte proliferation GO:0010839 IEA
    negative regulation of neuron projection development GO:0010977 IDA
    T cell costimulation GO:0031295 IEA
    adherens junction organization GO:0034332 IEA
    keratinocyte proliferation GO:0043616 IEA
    symbiont entry into host cell GO:0046718 IEA
    ephrin receptor signaling pathway GO:0048013 IBA
    ephrin receptor signaling pathway GO:0048013 IDA
    blood vessel morphogenesis GO:0048514 IBA
    venous blood vessel morphogenesis GO:0048845 IEA
    regulation of chemotaxis GO:0050920 IDA
    cellular response to lipopolysaccharide GO:0071222 IEA
    nephric duct morphogenesis GO:0072178 IEA
    presynapse assembly GO:0099054 IEA
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IEA
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IEA
    positive regulation of aorta morphogenesis GO:1903849 IEA
    positive regulation of cardiac muscle cell differentiation GO:2000727 ISS
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    adherens junction GO:0005912 IEA
    focal adhesion GO:0005925 HDA
    dendrite GO:0030425 IEA
    presynaptic membrane GO:0042734 IBA
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    postsynaptic density membrane GO:0098839 IEA
    glutamatergic synapse GO:0098978 IBA
 Experiment description of studies that identified EFNB2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for EFNB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GJD3  
Proximity Label-MS Homo sapiens
2 OCLN 100506658
Proximity Label-MS Homo sapiens
3 Ephb1  
Reconstituted Complex Rattus norvegicus
4 MARCKS 4082
Proximity Label-MS Homo sapiens
5 LAMP3  
Proximity Label-MS Homo sapiens
6 GJA1 2697
Proximity Label-MS Homo sapiens
7 PKP2 5318
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PARD3 56288
Proximity Label-MS Homo sapiens
9 EIF2B2 8892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CAND2 23066
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LAMP2 3920
Proximity Label-MS Homo sapiens
12 STX4 6810
Proximity Label-MS Homo sapiens
13 PLEKHG4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 B3GAT1  
Proximity Label-MS Homo sapiens
15 RDX 5962
Proximity Label-MS Homo sapiens
16 MLLT4 4301
Proximity Label-MS Homo sapiens
17 USO1 8615
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 FAM171A2 284069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SLC39A11  
Affinity Capture-MS Homo sapiens
20 PNPLA6 10908
Affinity Capture-MS Homo sapiens
21 NUS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 STX6 10228
Proximity Label-MS Homo sapiens
23 CD28  
Affinity Capture-MS Homo sapiens
24 RAB35 11021
Proximity Label-MS Homo sapiens
25 EBAG9 9166
Proximity Label-MS Homo sapiens
26 DPH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 FAM120A 23196
Affinity Capture-MS Homo sapiens
28 PLEKHH3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 LAMTOR1 55004
Proximity Label-MS Homo sapiens
30 METTL13 51603
Affinity Capture-MS Homo sapiens
31 EPHA5 2044
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 CXADR 1525
Proximity Label-MS Homo sapiens
33 EIF2B5 8893
Affinity Capture-MS Homo sapiens
34 LYN 4067
Proximity Label-MS Homo sapiens
35 DNAJC5 80331
Proximity Label-MS Homo sapiens
36 PAG1 55824
Affinity Capture-Western Homo sapiens
37 RGS3 5998
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 DIRAS3  
Proximity Label-MS Homo sapiens
39 TYK2 7297
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 TIGD5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 HPS3 84343
Affinity Capture-MS Homo sapiens
42 KIAA0391  
Affinity Capture-MS Homo sapiens
43 MTMR1 8776
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PIK3R1 5295
Affinity Capture-MS Homo sapiens
45 EPHB2 2048
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SEC61B 10952
Proximity Label-MS Homo sapiens
47 EIF2B4 8890
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 EPHB4 2050
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 PIK3CB 5291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 TEX2 55852
Affinity Capture-MS Homo sapiens
51 PHLPP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 PICK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 DIP2B 57609
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 TSPAN3 10099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 SDC3  
Affinity Capture-MS Homo sapiens
56 MRRF  
Affinity Capture-MS Homo sapiens
57 RAB5A 5868
Proximity Label-MS Homo sapiens
58 ATP2B2 491
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 LAMP1 3916
Proximity Label-MS Homo sapiens
60 DAD1 1603
Affinity Capture-MS Homo sapiens
61 DIP2A 23181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 LMBR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 ARF6 382
Proximity Label-MS Homo sapiens
64 AAAS 8086
Affinity Capture-MS Homo sapiens
65 SBF1 6305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 PKN3 29941
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 SDC2 6383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 TM9SF3 56889
Affinity Capture-MS Homo sapiens
69 RADIL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 SAAL1 113174
Affinity Capture-MS Homo sapiens
71 PI4K2B 55300
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 RETSAT 54884
Affinity Capture-MS Homo sapiens
73 PIK3CA 5290
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 RPN1 6184
Affinity Capture-MS Homo sapiens
75 RHOB 388
Proximity Label-MS Homo sapiens
76 EPHB3 2049
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 MAP7D2 256714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 DENND6A 201627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 KIF14 9928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 EPHA3 2042
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
81 TP53 7157
Negative Genetic Homo sapiens
82 EZR 7430
Proximity Label-MS Homo sapiens
83 LCK 3932
Proximity Label-MS Homo sapiens
84 EFNB1 1947
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 C11orf52 91894
Proximity Label-MS Homo sapiens
86 MAP7D3 79649
Affinity Capture-MS Homo sapiens
87 UBE3C 9690
Affinity Capture-MS Homo sapiens
88 MED16 10025
Affinity Capture-MS Homo sapiens
89 NF1 4763
Affinity Capture-MS Homo sapiens
90 RAB2A 5862
Proximity Label-MS Homo sapiens
91 ME1 4199
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 DNM3 26052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 RAB11A 8766
Proximity Label-MS Homo sapiens
94 EPHA2 1969
Proximity Label-MS Homo sapiens
95 PIK3R2 5296
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 KRAS 3845
Proximity Label-MS Homo sapiens
97 RAB5C 5878
Proximity Label-MS Homo sapiens
98 ANKRD46 157567
Two-hybrid Homo sapiens
99 POM121  
Affinity Capture-MS Homo sapiens
100 CAV1 857
Proximity Label-MS Homo sapiens
101 PIK3R3 8503
Affinity Capture-MS Homo sapiens
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