Gene description for Rfx3
Gene name regulatory factor X, 3 (influences HLA class II expression)
Gene symbol Rfx3
Other names/aliases C230093O12Rik
MRFX3
Species Mus musculus
 Database cross references - Rfx3
ExoCarta ExoCarta_19726
Vesiclepedia VP_19726
Entrez Gene 19726
UniProt P48381  
 Rfx3 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Rfx3
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    transcription cis-regulatory region binding GO:0000976 ISO
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity GO:0003700 IDA
    protein binding GO:0005515 IPI
    sequence-specific DNA binding GO:0043565 IDA
    sequence-specific double-stranded DNA binding GO:1990837 ISO
Biological Process
    DNA-templated transcription GO:0006351 IMP
    regulation of DNA-templated transcription GO:0006355 IMP
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    determination of left/right symmetry GO:0007368 IMP
    endocrine pancreas development GO:0031018 IMP
    negative regulation of DNA-templated transcription GO:0045892 ISO
    negative regulation of DNA-templated transcription GO:0045892 ISS
    positive regulation of DNA-templated transcription GO:0045893 IDA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    cell maturation GO:0048469 IMP
    regulation of insulin secretion GO:0050796 IMP
    cilium assembly GO:0060271 IMP
    cilium-dependent cell motility GO:0060285 IMP
    epithelial cilium movement involved in determination of left/right asymmetry GO:0060287 IMP
    type B pancreatic cell maturation GO:0072560 IMP
    positive regulation of type B pancreatic cell development GO:2000078 IMP
Subcellular Localization
    chromatin GO:0000785 IDA
    chromatin GO:0000785 ISO
    extracellular region GO:0005576 IEA
    nucleus GO:0005634 IEA
    transcription regulator complex GO:0005667 IDA
 Experiment description of studies that identified Rfx3 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Rfx3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rfx3 is involved
No pathways found





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