Gene description for St3gal4
Gene name ST3 beta-galactoside alpha-2,3-sialyltransferase 4
Gene symbol St3gal4
Other names/aliases Siat4c
Species Mus musculus
 Database cross references - St3gal4
ExoCarta ExoCarta_20443
Vesiclepedia VP_20443
Entrez Gene 20443
UniProt Q91Y74  
 St3gal4 identified in sEVs derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
Melanoma cells 34957415    
 Gene ontology annotations for St3gal4
Molecular Function
    beta-galactoside (CMP) alpha-2,3-sialyltransferase activity GO:0003836 IBA
    beta-galactoside (CMP) alpha-2,3-sialyltransferase activity GO:0003836 IDA
    beta-galactoside (CMP) alpha-2,3-sialyltransferase activity GO:0003836 ISO
    N-acetyllactosaminide alpha-2,3-sialyltransferase activity GO:0008118 IMP
    N-acetyllactosaminide alpha-2,3-sialyltransferase activity GO:0008118 ISO
    N-acetyllactosaminide alpha-2,3-sialyltransferase activity GO:0008118 ISS
    beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminide alpha-2,3- sialyltransferase GO:0047288 IBA
    beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminide alpha-2,3- sialyltransferase GO:0047288 IEA
    beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminide alpha-2,3- sialyltransferase GO:0047288 ISO
Biological Process
    protein glycosylation GO:0006486 IBA
    protein glycosylation GO:0006486 IDA
    protein glycosylation GO:0006486 IEA
    protein glycosylation GO:0006486 ISO
    glycoprotein biosynthetic process GO:0009101 ISO
    glycolipid biosynthetic process GO:0009247 IBA
    glycolipid biosynthetic process GO:0009247 IEA
    glycolipid biosynthetic process GO:0009247 ISO
    oligosaccharide biosynthetic process GO:0009312 IEA
    oligosaccharide biosynthetic process GO:0009312 ISO
    positive regulation of blood coagulation GO:0030194 IMP
    lipid glycosylation GO:0030259 IEA
    lipid glycosylation GO:0030259 ISO
    cognition GO:0050890 IEA
    cognition GO:0050890 ISO
    sialylation GO:0097503 IEA
    sialylation GO:0097503 ISO
    positive regulation of leukocyte tethering or rolling GO:1903238 IMP
    positive regulation of leukocyte tethering or rolling GO:1903238 ISO
    protein sialylation GO:1990743 ISO
Subcellular Localization
    Golgi cisterna membrane GO:0032580 IEA
 Experiment description of studies that identified St3gal4 in sEVs
1
Experiment ID 1101
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1102
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F10
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
3
Experiment ID 1103
MISEV standards
✔
EM
Biophysical techniques
✔
Cd81|Cd9|Tfrc|Gapdh|Lamp1
Enriched markers
✔
GOLGA2|CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for St3gal4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Brap  
Co-fractionation Mus musculus
View the network image/svg+xml



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