Gene description for Sparc
Gene name secreted acidic cysteine rich glycoprotein
Gene symbol Sparc
Other names/aliases BM-40
ON
Species Mus musculus
 Database cross references - Sparc
ExoCarta ExoCarta_20692
Vesiclepedia VP_20692
Entrez Gene 20692
UniProt P07214  
 Sparc identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Sparc
Molecular Function
    extracellular matrix structural constituent GO:0005201 RCA
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IDA
    calcium ion binding GO:0005509 ISO
    protein binding GO:0005515 IPI
    collagen binding GO:0005518 IBA
    collagen binding GO:0005518 ISO
    extracellular matrix binding GO:0050840 IBA
    extracellular matrix binding GO:0050840 IDA
Biological Process
    negative regulation of endothelial cell proliferation GO:0001937 ISO
    positive regulation of endothelial cell migration GO:0010595 ISO
    negative regulation of angiogenesis GO:0016525 ISO
    regulation of cell morphogenesis GO:0022604 ISO
    lung development GO:0030324 ISO
    regulation of cell population proliferation GO:0042127 IGI
    pigmentation GO:0043473 IMP
    semicircular canal morphogenesis GO:0048752 IBA
    regulation of synapse organization GO:0050807 IBA
    regulation of synapse organization GO:0050807 ISO
    bone development GO:0060348 IMP
    cellular response to growth factor stimulus GO:0071363 IDA
Subcellular Localization
    basement membrane GO:0005604 IEA
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 ISO
    cell surface GO:0009986 ISO
    nuclear matrix GO:0016363 ISO
    extracellular matrix GO:0031012 IDA
    platelet alpha granule GO:0031091 ISO
    platelet alpha granule membrane GO:0031092 ISO
    vesicle GO:0031982 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    synapse GO:0045202 ISO
    collagen-containing extracellular matrix GO:0062023 HDA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Sparc in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sparc
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tgm2  
Co-fractionation Mus musculus
2 Apc  
Phenotypic Suppression Mus musculus
3 Kcnma1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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