Gene description for Spp1
Gene name secreted phosphoprotein 1
Gene symbol Spp1
Other names/aliases 2AR
Apl-1
BNSP
BSPI
Bsp
ETA-1
Eta
OP
Opn
Opnl
Ric
Spp-1
Species Mus musculus
 Database cross references - Spp1
ExoCarta ExoCarta_20750
Vesiclepedia VP_20750
Entrez Gene 20750
UniProt P10923  
 Spp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Spp1
Molecular Function
    cytokine activity GO:0005125 NAS
    integrin binding GO:0005178 IDA
    integrin binding GO:0005178 ISO
    small molecule binding GO:0036094 ISO
    extracellular matrix binding GO:0050840 IBA
    extracellular matrix binding GO:0050840 IDA
Biological Process
    osteoblast differentiation GO:0001649 IBA
    osteoblast differentiation GO:0001649 ISO
    androgen catabolic process GO:0006710 ISO
    intracellular calcium ion homeostasis GO:0006874 IMP
    intracellular sodium ion homeostasis GO:0006883 IMP
    cell adhesion GO:0007155 IBA
    cell adhesion GO:0007155 IEA
    cell adhesion GO:0007155 ISO
    signal transduction GO:0007165 IEA
    positive regulation of cell-substrate adhesion GO:0010811 IDA
    bone mineralization GO:0030282 IDA
    bone mineralization GO:0030282 IMP
    neutrophil chemotaxis GO:0030593 IDA
    neutrophil chemotaxis GO:0030593 IGI
    intracellular phosphate ion homeostasis GO:0030643 IMP
    intracellular chloride ion homeostasis GO:0030644 IMP
    response to vitamin D GO:0033280 ISO
    urate biosynthetic process GO:0034418 IMP
    negative regulation of apoptotic process GO:0043066 NAS
    positive regulation of bone resorption GO:0045780 IBA
    positive regulation of bone resorption GO:0045780 ISO
    positive regulation of DNA-templated transcription GO:0045893 ISO
    response to steroid hormone GO:0048545 ISO
    calcium ion homeostasis GO:0055074 IDA
    cellular response to testosterone stimulus GO:0071394 ISO
    cellular response to fluid shear stress GO:0071498 IDA
    collecting duct development GO:0072044 IMP
    cellular response to leukemia inhibitory factor GO:1990830 IEP
    positive regulation of estradiol secretion GO:2000866 ISO
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 ISO
    vesicle GO:0031982 ISO
    cell projection GO:0042995 ISO
    apical part of cell GO:0045177 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
 Experiment description of studies that identified Spp1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Spp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Bcl6  
Affinity Capture-Western Mus musculus
2 Usp7  
Affinity Capture-MS Mus musculus
3 Cdkn2a  
Two-hybrid Mus musculus
4 Runx2  
Affinity Capture-MS Mus musculus
5 Fth1 14319
Co-fractionation Mus musculus
6 Pik3r1  
Affinity Capture-Western Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here