Gene description for Sqle
Gene name squalene epoxidase
Gene symbol Sqle
Other names/aliases AI323792
Species Mus musculus
 Database cross references - Sqle
ExoCarta ExoCarta_20775
Vesiclepedia VP_20775
Entrez Gene 20775
UniProt P52019  
 Sqle identified in sEVs derived from the following tissue/cell type
Neural stem cells 25242146    
 Gene ontology annotations for Sqle
Molecular Function
    squalene monooxygenase activity GO:0004506 IBA
    squalene monooxygenase activity GO:0004506 ISO
    squalene monooxygenase activity GO:0004506 ISS
    FAD binding GO:0071949 ISO
    FAD binding GO:0071949 ISS
Biological Process
    cholesterol metabolic process GO:0008203 IBA
    cholesterol metabolic process GO:0008203 IEA
    cholesterol metabolic process GO:0008203 ISO
    sterol biosynthetic process GO:0016126 IBA
    sterol biosynthetic process GO:0016126 ISO
    sterol biosynthetic process GO:0016126 ISS
    regulation of cell population proliferation GO:0042127 ISO
    regulation of cell population proliferation GO:0042127 ISS
    lipid droplet formation GO:0140042 ISO
    lipid droplet formation GO:0140042 ISS
    response to biphenyl GO:1904614 IEA
    response to biphenyl GO:1904614 ISO
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 IEA
    membrane GO:0016020 ISO
    membrane GO:0016020 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
    intracellular membrane-bounded organelle GO:0043231 ISS
 Experiment description of studies that identified Sqle in sEVs
1
Experiment ID 263
MISEV standards
Biophysical techniques
CD63|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Sqle
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tmem173  
Proximity Label-MS Mus musculus
View the network image/svg+xml
 Pathways in which Sqle is involved
PathwayEvidenceSource
Cholesterol biosynthesis IEA Reactome
Metabolism IEA Reactome
Metabolism of lipids IEA Reactome
Metabolism of steroids IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here