Gene description for Slk
Gene name STE20-like kinase
Gene symbol Slk
Other names/aliases 9A2
AV021402
AW411554
Etk4
SMAK
Stk2
mKIAA0204
mSLK
Species Mus musculus
 Database cross references - Slk
ExoCarta ExoCarta_20874
Entrez Gene 20874
UniProt O54988  
 Slk identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
 Gene ontology annotations for Slk
Molecular Function
    protein homodimerization activity GO:0042803 ISO
    protein kinase activity GO:0004672 ISO
    protein serine/threonine kinase activity GO:0004674 ISO
    transferase activity GO:0016740 IEA
    nucleotide binding GO:0000166 IEA
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    kinase activity GO:0016301 IEA
    ATP binding GO:0005524 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    signal transduction by protein phosphorylation GO:0023014 IBA
    positive regulation of histone phosphorylation GO:0033129 ISO
    stress-activated protein kinase signaling cascade GO:0031098 IBA
    activation of protein kinase activity GO:0032147 IBA
    positive regulation of apoptotic process GO:0043065 ISO
    phosphorylation GO:0016310 IEA
    regulation of mitotic cell cycle GO:0007346 IBA
    regulation of apoptotic process GO:0042981 IBA
    protein phosphorylation GO:0006468 ISO
    apoptotic process GO:0006915 IEA
    protein autophosphorylation GO:0046777 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Slk in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Slk
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Slk is involved
No pathways found





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