Gene description for Pycr1
Gene name pyrroline-5-carboxylate reductase 1
Gene symbol Pycr1
Other names/aliases -
Species Mus musculus
 Database cross references - Pycr1
ExoCarta ExoCarta_209027
Vesiclepedia VP_209027
Entrez Gene 209027
UniProt Q922W5  
 Pycr1 identified in sEVs derived from the following tissue/cell type
Neural stem cells 25242146    
 Gene ontology annotations for Pycr1
Molecular Function
    pyrroline-5-carboxylate reductase activity GO:0004735 IBA
    pyrroline-5-carboxylate reductase activity GO:0004735 ISO
    pyrroline-5-carboxylate reductase activity GO:0004735 ISS
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    proline biosynthetic process GO:0006561 ISO
    proline biosynthetic process GO:0006561 ISS
    cellular response to oxidative stress GO:0034599 ISO
    cellular response to oxidative stress GO:0034599 ISS
    regulation of mitochondrial membrane potential GO:0051881 IEA
    regulation of mitochondrial membrane potential GO:0051881 ISO
    L-proline biosynthetic process GO:0055129 IBA
    L-proline biosynthetic process GO:0055129 IEA
    negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway GO:1903377 IEA
    negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway GO:1903377 ISO
Subcellular Localization
    mitochondrion GO:0005739 HDA
    mitochondrion GO:0005739 ISO
    mitochondrion GO:0005739 ISS
 Experiment description of studies that identified Pycr1 in sEVs
1
Experiment ID 264
MISEV standards
Biophysical techniques
CD63|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th2 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pycr1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Atg16l1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Pycr1 is involved
PathwayEvidenceSource
Glutamate and glutamine metabolism IEA Reactome
Metabolism IEA Reactome
Metabolism of amino acids and derivatives IEA Reactome





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