Gene description for Hk3
Gene name hexokinase 3
Gene symbol Hk3
Other names/aliases HK III
HK-III
Species Mus musculus
 Database cross references - Hk3
ExoCarta ExoCarta_212032
Entrez Gene 212032
UniProt Q3TRM8  
 Hk3 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Hk3
Molecular Function
    nucleotide binding GO:0000166 IEA
    phosphotransferase activity, alcohol group as acceptor GO:0016773 IEA
    kinase activity GO:0016301 IEA
    glucokinase activity GO:0004340 IBA
    ATP binding GO:0005524 IEA
    mannokinase activity GO:0019158 IBA
    hormone binding GO:0042562 ISO
    enzyme binding GO:0019899 ISO
    catalytic activity GO:0003824 IEA
    transferase activity GO:0016740 IEA
    fructokinase activity GO:0008865 IBA
    hexokinase activity GO:0004396 ISO
Biological Process
    carbohydrate phosphorylation GO:0046835 ISO
    cellular glucose homeostasis GO:0001678 IBA
    glycolytic process GO:0006096 IBA
    carbohydrate metabolic process GO:0005975 IEA
    negative regulation of hydrogen peroxide-mediated programmed cell death GO:1901299 ISO
    metabolic process GO:0008152 IEA
    glucose 6-phosphate metabolic process GO:0051156 IBA
    phosphorylation GO:0016310 IEA
Subcellular Localization
    mitochondrion GO:0005739 IDA
    protein complex GO:0043234 ISO
    cytosol GO:0005829 IBA
 Experiment description of studies that identified Hk3 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Hk3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hk3 is involved
PathwayEvidenceSource
Glucose transport IEA Reactome
Glycolysis IEA Reactome





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