Gene description for Ago3
Gene name argonaute RISC catalytic subunit 3
Gene symbol Ago3
Other names/aliases AW048688
C130014L07Rik
Eif2c3
Species Mus musculus
 Database cross references - Ago3
ExoCarta ExoCarta_214150
Entrez Gene 214150
UniProt Q8CJF9  
 Ago3 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ago3
Molecular Function
    nucleic acid binding GO:0003676 IEA
    pre-miRNA binding GO:0070883 ISO
    single-stranded RNA binding GO:0003727 ISO
    poly(A) RNA binding GO:0044822 ISO
    RNA binding GO:0003723 IDA
    miRNA binding GO:0035198 ISO
    double-stranded RNA binding GO:0003725 ISO
Biological Process
    mRNA catabolic process GO:0006402 ISO
    negative regulation of translation involved in gene silencing by miRNA GO:0035278 ISO
    gene silencing by RNA GO:0031047 IEA
    RNA secondary structure unwinding GO:0010501 ISO
    regulation of stem cell proliferation GO:0072091 ISO
    miRNA loading onto RISC involved in gene silencing by miRNA GO:0035280 ISO
    pre-miRNA processing GO:0031054 ISO
    regulation of translation GO:0006417 IEA
    production of miRNAs involved in gene silencing by miRNA GO:0035196 ISO
Subcellular Localization
    membrane GO:0016020 ISO
    RISC complex GO:0016442 ISO
    ribonucleoprotein complex GO:0030529 IEA
    RISC-loading complex GO:0070578 ISO
    micro-ribonucleoprotein complex GO:0035068 IDA
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Ago3 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ago3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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