Gene description for Tcn2
Gene name transcobalamin 2
Gene symbol Tcn2
Other names/aliases AW208754
Tcn-2
Species Mus musculus
 Database cross references - Tcn2
ExoCarta ExoCarta_21452
Vesiclepedia VP_21452
Entrez Gene 21452
UniProt O88968  
 Tcn2 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Tcn2
Molecular Function
    cobalamin binding GO:0031419 IBA
    cobalamin binding GO:0031419 IDA
    cobalamin binding GO:0031419 ISO
    cobalamin binding GO:0031419 ISS
    metal ion binding GO:0046872 IEA
    molecular carrier activity GO:0140104 IDA
    cargo receptor ligand activity GO:0140355 IDA
    cargo receptor ligand activity GO:0140355 ISO
Biological Process
    cobalt ion transport GO:0006824 IEA
    cobalamin transport GO:0015889 IBA
    cobalamin transport GO:0015889 IDA
    cobalamin transport GO:0015889 ISO
    cobalamin transport GO:0015889 ISS
Subcellular Localization
    extracellular space GO:0005615 HDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISS
    external side of plasma membrane GO:0009897 IDA
 Experiment description of studies that identified Tcn2 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Tcn2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zbtb7b  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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