Gene description for Gemin5
Gene name gem (nuclear organelle) associated protein 5
Gene symbol Gemin5
Other names/aliases AA407055
AA407208
AI451603
BB194447
C330013N08
Species Mus musculus
 Database cross references - Gemin5
ExoCarta ExoCarta_216766
Vesiclepedia VP_216766
Entrez Gene 216766
UniProt Q8BX17  
 Gemin5 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Gemin5
Molecular Function
    RNA 7-methylguanosine cap binding GO:0000340 ISO
    RNA 7-methylguanosine cap binding GO:0000340 ISS
    mRNA 3'-UTR binding GO:0003730 IBA
    mRNA 3'-UTR binding GO:0003730 IDA
    mRNA 3'-UTR binding GO:0003730 ISO
    snRNA binding GO:0017069 ISO
    snRNA binding GO:0017069 ISS
    U1 snRNA binding GO:0030619 ISO
    U1 snRNA binding GO:0030619 ISS
    U4 snRNA binding GO:0030621 ISO
    U4 snRNA binding GO:0030621 ISS
    U4atac snRNA binding GO:0030622 ISO
    ribosome binding GO:0043022 ISO
    ribosome binding GO:0043022 ISS
Biological Process
    spliceosomal snRNP assembly GO:0000387 IBA
    spliceosomal snRNP assembly GO:0000387 ISO
    spliceosomal snRNP assembly GO:0000387 ISS
    translation GO:0006412 IEA
    regulation of translation GO:0006417 ISO
    regulation of translation GO:0006417 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    nuclear body GO:0016604 ISO
    SMN complex GO:0032797 IBA
    SMN complex GO:0032797 ISO
    SMN complex GO:0032797 ISS
    SMN-Gemin2 complex GO:0034718 ISO
    SMN-Gemin2 complex GO:0034718 ISS
    SMN-Sm protein complex GO:0034719 ISO
    SMN-Sm protein complex GO:0034719 ISS
    Gemini of coiled bodies GO:0097504 ISS
 Experiment description of studies that identified Gemin5 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Gemin5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zbtb7b  
Affinity Capture-MS Mus musculus
2 Atg16l1  
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Atg5  
Affinity Capture-RNA Mus musculus
5 Map3k5  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Gemin5 is involved
PathwayEvidenceSource
Metabolism of non-coding RNA IEA Reactome
Metabolism of RNA IEA Reactome
snRNP Assembly IEA Reactome





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