Gene description for Tph1
Gene name tryptophan hydroxylase 1
Gene symbol Tph1
Other names/aliases Tph
Species Mus musculus
 Database cross references - Tph1
ExoCarta ExoCarta_21990
Entrez Gene 21990
UniProt P17532  
 Tph1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Tph1
Molecular Function
    iron ion binding GO:0005506 IEA
    oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced pteridine as one donor, and incorporation of one atom of oxygen GO:0016714 IEA
    amino acid binding GO:0016597 IEA
    oxidoreductase activity GO:0016491 IEA
    metal ion binding GO:0046872 IEA
    tryptophan 5-monooxygenase activity GO:0004510 ISO
    monooxygenase activity GO:0004497 IEA
Biological Process
    negative regulation of ossification GO:0030279 ISO
    serotonin biosynthetic process GO:0042427 IEA
    metabolic process GO:0008152 IEA
    oxidation-reduction process GO:0055114 ISO
    bone remodeling GO:0046849 IGI
    positive regulation of fat cell differentiation GO:0045600 IMP
    mammary gland alveolus development GO:0060749 IMP
    aromatic amino acid family metabolic process GO:0009072 IEA
Subcellular Localization
    neuron projection GO:0043005 ISO
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified Tph1 in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Tph1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tph1 is involved
PathwayEvidenceSource
Serotonin and melatonin biosynthesis IEA Reactome





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