Gene description for Traf4
Gene name TNF receptor associated factor 4
Gene symbol Traf4
Other names/aliases A530032M13Rik
CART1
msp2
Species Mus musculus
 Database cross references - Traf4
ExoCarta ExoCarta_22032
Vesiclepedia VP_22032
Entrez Gene 22032
UniProt Q61382  
 Traf4 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Traf4
Molecular Function
    tumor necrosis factor receptor binding GO:0005164 IEA
    tumor necrosis factor receptor binding GO:0005164 ISO
    zinc ion binding GO:0008270 IEA
    transferase activity GO:0016740 IEA
    protein kinase binding GO:0019901 IPI
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    thioesterase binding GO:0031996 IEA
    thioesterase binding GO:0031996 ISO
    signaling adaptor activity GO:0035591 IBA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    WW domain binding GO:0050699 IEA
    WW domain binding GO:0050699 ISO
Biological Process
    apoptotic process GO:0006915 IEA
    signal transduction GO:0007165 IEA
    cell surface receptor signaling pathway GO:0007166 IBA
    respiratory gaseous exchange by respiratory system GO:0007585 IMP
    respiratory tube development GO:0030323 IMP
    regulation of apoptotic process GO:0042981 IEA
    regulation of canonical NF-kappaB signal transduction GO:0043122 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    innate immune response GO:0045087 IEA
    positive regulation of protein kinase activity GO:0045860 ISO
    positive regulation of protein kinase activity GO:0045860 ISS
    positive regulation of JNK cascade GO:0046330 ISO
    positive regulation of JNK cascade GO:0046330 ISS
Subcellular Localization
    fibrillar center GO:0001650 IEA
    fibrillar center GO:0001650 ISO
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    cytoskeleton GO:0005856 IEA
    plasma membrane GO:0005886 IEA
    bicellular tight junction GO:0005923 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
    plasma membrane signaling receptor complex GO:0098802 IBA
 Experiment description of studies that identified Traf4 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Traf4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Map3k4  
Affinity Capture-Western Mus musculus
2 Ngfrap1  
Co-fractionation Mus musculus
3 Traf3ip2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
4 Fhl2  
Affinity Capture-Western Mus musculus
5 Mrpl28  
Co-fractionation Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
7 Ubc  
Reconstituted Complex Mus musculus
View the network image/svg+xml
 Pathways in which Traf4 is involved
No pathways found





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