Gene description for Plscr1
Gene name phospholipid scramblase 1
Gene symbol Plscr1
Other names/aliases MmTRA1a
MmTRA1b
Nor1
Tra1
Tra1a
Tra1b
Tras1
Tras2
Species Mus musculus
 Database cross references - Plscr1
ExoCarta ExoCarta_22038
Entrez Gene 22038
UniProt Q9JJ00  
 Plscr1 identified in exosomes derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Mast cells 17486113    
 Gene ontology annotations for Plscr1
Molecular Function
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 ISO
    epidermal growth factor receptor binding GO:0005154 ISO
    enzyme binding GO:0019899 ISO
    SH3 domain binding GO:0017124 ISO
    calcium ion binding GO:0005509 ISO
    phospholipid scramblase activity GO:0017128 ISO
    CD4 receptor binding GO:0042609 ISO
    DNA binding GO:0003677 IEA
Biological Process
    phosphatidylserine biosynthetic process GO:0006659 IDA
    response to interferon-alpha GO:0035455 IMP
    negative regulation of viral genome replication GO:0045071 ISO
    response to interferon-beta GO:0035456 ISO
    phosphatidylserine exposure on apoptotic cell surface GO:0070782 IDA
    regulation of Fc receptor mediated stimulatory signaling pathway GO:0060368 ISO
    negative regulation of protein binding GO:0032091 IDA
    regulation of mast cell activation GO:0033003 ISO
    phospholipid transport GO:0015914 IBA
    defense response to virus GO:0051607 ISO
    phospholipid scrambling GO:0017121 ISO
    intrinsic apoptotic signaling pathway GO:0097193 IDA
    positive regulation of gene expression GO:0010628 ISO
    myeloid cell differentiation GO:0030099 IMP
    immune response GO:0006955 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    apoptotic process GO:0006915 ISO
    positive regulation of innate immune response GO:0045089 ISO
    positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity GO:2000373 ISO
    positive regulation of apoptotic process GO:0043065 IGI
    positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage GO:1902231 IDA
    transcription from RNA polymerase II promoter GO:0006366 ISO
Subcellular Localization
    membrane GO:0016020 ISO
    extracellular matrix GO:0031012 ISO
    membrane raft GO:0045121 ISO
    integral component of plasma membrane GO:0005887 ISO
    Golgi apparatus GO:0005794 ISO
    nucleus GO:0005634 ISO
    extracellular exosome GO:0070062 ISO
    plasma membrane GO:0005886 ISO
    nucleolus GO:0005730 ISO
    cytosol GO:0005829 ISO
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified Plscr1 in exosomes
1
Experiment ID 89
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M.
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Plscr1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Plscr1 is involved
No pathways found





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