Gene description for Plscr1
Gene name phospholipid scramblase 1
Gene symbol Plscr1
Other names/aliases MmTRA1a
MmTRA1b
Nor1
Tra1
Tra1a
Tra1b
Tras1
Tras2
Species Mus musculus
 Database cross references - Plscr1
ExoCarta ExoCarta_22038
Vesiclepedia VP_22038
Entrez Gene 22038
UniProt Q9JJ00  
 Plscr1 identified in sEVs derived from the following tissue/cell type
Basophilic leukemia cells 20424270    
Colon cancer cells 37309723    
Faeces 33431899    
Faeces 33431899    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Plscr1
Molecular Function
    magnesium ion binding GO:0000287 ISO
    magnesium ion binding GO:0000287 ISS
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 ISO
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 ISS
    virus receptor activity GO:0001618 ISO
    DNA binding GO:0003677 IEA
    nuclease activity GO:0004518 ISO
    nuclease activity GO:0004518 ISS
    epidermal growth factor receptor binding GO:0005154 ISO
    epidermal growth factor receptor binding GO:0005154 ISS
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    SH3 domain binding GO:0017124 ISO
    SH3 domain binding GO:0017124 ISS
    phospholipid scramblase activity GO:0017128 IBA
    phospholipid scramblase activity GO:0017128 ISO
    phospholipid scramblase activity GO:0017128 ISS
    enzyme binding GO:0019899 ISO
    lead ion binding GO:0032791 ISO
    lead ion binding GO:0032791 ISS
    CD4 receptor binding GO:0042609 ISO
    CD4 receptor binding GO:0042609 ISS
    mercury ion binding GO:0045340 ISO
    mercury ion binding GO:0045340 ISS
Biological Process
    phosphatidylserine biosynthetic process GO:0006659 IDA
    apoptotic process GO:0006915 ISO
    acute-phase response GO:0006953 IEP
    immune response GO:0006955 ISO
    response to lead ion GO:0010288 ISO
    positive regulation of gene expression GO:0010628 ISO
    plasma membrane phospholipid scrambling GO:0017121 IDA
    plasma membrane phospholipid scrambling GO:0017121 ISO
    myeloid cell differentiation GO:0030099 IMP
    negative regulation of protein binding GO:0032091 IDA
    regulation of mast cell activation GO:0033003 ISO
    regulation of mast cell activation GO:0033003 ISS
    response to interferon-alpha GO:0035455 IMP
    response to interferon-beta GO:0035456 ISO
    positive regulation of apoptotic process GO:0043065 IGI
    negative regulation of viral genome replication GO:0045071 IMP
    negative regulation of viral genome replication GO:0045071 ISO
    positive regulation of innate immune response GO:0045089 IMP
    positive regulation of innate immune response GO:0045089 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    negative regulation of phagocytosis GO:0050765 ISO
    negative regulation of phagocytosis GO:0050765 ISS
    defense response to virus GO:0051607 IMP
    defense response to virus GO:0051607 ISO
    regulation of Fc receptor mediated stimulatory signaling pathway GO:0060368 ISO
    regulation of Fc receptor mediated stimulatory signaling pathway GO:0060368 ISS
    phosphatidylserine exposure on apoptotic cell surface GO:0070782 IBA
    phosphatidylserine exposure on apoptotic cell surface GO:0070782 IDA
    phosphatidylserine exposure on apoptotic cell surface GO:0070782 IMP
    phosphatidylserine exposure on apoptotic cell surface GO:0070782 ISO
    cellular response to lipopolysaccharide GO:0071222 IEP
    cellular response to cytokine stimulus GO:0071345 IEP
    intrinsic apoptotic signaling pathway GO:0097193 IDA
    positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage GO:1902231 IDA
    positive regulation of chromosome separation GO:1905820 ISO
    positive regulation of chromosome separation GO:1905820 ISS
    positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity GO:2000373 ISO
    positive regulation of DNA topoisomerase (ATP-hydrolyzing) activity GO:2000373 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 ISO
    nucleolus GO:0005730 ISS
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    membrane raft GO:0045121 ISO
    membrane raft GO:0045121 ISS
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
    collagen-containing extracellular matrix GO:0062023 ISO
    collagen-containing extracellular matrix GO:0062023 ISS
 Experiment description of studies that identified Plscr1 in sEVs
1
Experiment ID 89
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20424270    
Organism Rattus norvegicus
Experiment description Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins.
Authors "Subra C, Grand D, Laulagnier K, Stella A, Lambeau G, Paillasse M, De Medina P, Monsarrat B, Perret B, Silvente-Poirot S, Poirot M, Record M."
Journal name JLR
Publication year 2010
Sample Basophilic leukemia cells
Sample name RBL-2H3
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Gas chromatography
Thin layer chromatography
Mass spectrometry [Qstar XL]
FACS
2
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
4
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
5
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Plscr1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Trim59  
Two-hybrid Mus musculus
2 Rasd1  
Two-hybrid Mus musculus
View the network image/svg+xml
 Pathways in which Plscr1 is involved
No pathways found





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