Gene description for Prss2
Gene name protease, serine 2
Gene symbol Prss2
Other names/aliases TRY8
TRYP
Ta
Tesp4
Try2
Species Mus musculus
 Database cross references - Prss2
ExoCarta ExoCarta_22072
Vesiclepedia VP_22072
Entrez Gene 22072
UniProt P07146  
 Prss2 identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Prss2
Molecular Function
    serine-type endopeptidase activity GO:0004252 IBA
    serine-type endopeptidase activity GO:0004252 IDA
    serine-type endopeptidase activity GO:0004252 ISO
    serine-type endopeptidase activity GO:0004252 ISS
    calcium ion binding GO:0005509 ISO
    calcium ion binding GO:0005509 ISS
Biological Process
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 ISO
    proteolysis GO:0006508 ISS
    response to nutrient GO:0007584 IEA
    digestion GO:0007586 IEA
    collagen catabolic process GO:0030574 ISO
    collagen catabolic process GO:0030574 ISS
    response to caffeine GO:0031000 IEA
    response to caffeine GO:0031000 ISO
    response to nicotine GO:0035094 IEA
    response to nicotine GO:0035094 ISO
    response to acetaldehyde GO:1905640 IEA
Subcellular Localization
    extracellular region GO:0005576 ISO
    extracellular region GO:0005576 ISS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 ISO
    extracellular space GO:0005615 ISS
    collagen-containing extracellular matrix GO:0062023 HDA
 Experiment description of studies that identified Prss2 in sEVs
1
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Prss2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Eed  
Affinity Capture-MS Mus musculus
2 Defa26  
Co-fractionation Mus musculus
View the network image/svg+xml



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