Gene description for Wt1
Gene name Wilms tumor 1 homolog
Gene symbol Wt1
Other names/aliases D630046I19Rik
Wt-1
Species Mus musculus
 Database cross references - Wt1
ExoCarta ExoCarta_22431
Entrez Gene 22431
 Wt1 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Wt1
Molecular Function
    C2H2 zinc finger domain binding GO:0070742 ISS
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    sequence-specific DNA binding GO:0043565 ISS
    nucleic acid binding GO:0003676 IEA
    double-stranded DNA binding GO:0003690 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 IDA
    transcription regulatory region DNA binding GO:0044212 ISO
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 IDA
    RNA binding GO:0003723 IDA
    metal ion binding GO:0046872 IEA
    zinc ion binding GO:0008270 ISO
Biological Process
    glomerular visceral epithelial cell differentiation GO:0072112 IDA
    negative regulation of transcription, DNA-templated GO:0045892 ISS
    Sertoli cell development GO:0060009 IMP
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISO
    adrenal cortex formation GO:0035802 IMP
    branching involved in ureteric bud morphogenesis GO:0001658 ISO
    diaphragm development GO:0060539 IMP
    glomerulus development GO:0032835 ISO
    mesenchymal to epithelial transition GO:0060231 IMP
    vasculogenesis GO:0001570 IMP
    ureteric bud development GO:0001657 IMP
    male genitalia development GO:0030539 IMP
    metanephros development GO:0001656 IMP
    transcription from RNA polymerase II promoter GO:0006366 IDA
    sperm motility GO:0030317 IMP
    cellular response to gonadotropin stimulus GO:0071371 ISO
    metanephric S-shaped body morphogenesis GO:0072284 ISO
    epithelial cell differentiation GO:0030855 IMP
    negative regulation of mesenchymal cell apoptotic process involved in metanephros development GO:1900212 IMP
    regulation of transcription from RNA polymerase II promoter GO:0006357 IDA
    thorax and anterior abdomen determination GO:0007356 IMP
    adrenal gland development GO:0030325 ISO
    visceral serous pericardium development GO:0061032 TAS
    seminiferous tubule development GO:0072520 IMP
    negative regulation of cell growth GO:0030308 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of male gonad development GO:2000020 IMP
    germ cell development GO:0007281 IMP
    cardiac muscle cell fate commitment GO:0060923 IMP
    positive regulation of transcription, DNA-templated GO:0045893 ISO
    single fertilization GO:0007338 IMP
    regulation of transcription, DNA-templated GO:0006355 IDA
    kidney development GO:0001822 ISO
    negative regulation of cell proliferation GO:0008285 ISO
    apoptotic process GO:0006915 IMP
    cellular response to cAMP GO:0071320 IBA
    camera-type eye development GO:0043010 IMP
    regulation of gene expression GO:0010468 IMP
    regulation of organ formation GO:0003156 IMP
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    negative regulation of translation GO:0017148 ISO
    negative regulation of metanephric glomerular mesangial cell proliferation GO:0072302 IMP
    sex determination GO:0007530 ISO
    metanephric mesenchyme development GO:0072075 IMP
    coronary vasculature development GO:0060976 TAS
    positive regulation of metanephric ureteric bud development GO:2001076 IMP
    transcription, DNA-templated GO:0006351 IEA
    glomerular basement membrane development GO:0032836 ISO
    tissue development GO:0009888 IMP
    gonad development GO:0008406 IMP
    mesonephros development GO:0001823 IMP
    male gonad development GO:0008584 IMP
    spermatogenesis GO:0007283 IMP
    RNA splicing GO:0008380 IDA
    positive regulation of apoptotic process GO:0043065 ISO
    heart development GO:0007507 ISO
    negative regulation of female gonad development GO:2000195 IMP
    positive regulation of heart growth GO:0060421 IMP
    posterior mesonephric tubule development GO:0072166 IMP
Subcellular Localization
    cytoplasm GO:0005737 IDA
    nuclear speck GO:0016607 TAS
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Wt1 in exosomes
1
Experiment ID 15
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Wt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Wt1 is involved
No pathways found





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