Gene description for Bag6
Gene name BCL2-associated athanogene 6
Gene symbol Bag6
Other names/aliases 2410045D21Rik
AA408914
BAG-6
Bat3
D17H6S52E
G3
Scythe
Species Mus musculus
 Database cross references - Bag6
ExoCarta ExoCarta_224727
Vesiclepedia VP_224727
Entrez Gene 224727
UniProt Q9Z1R2  
 Bag6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Bag6
Molecular Function
    signaling receptor binding GO:0005102 ISO
    protein binding GO:0005515 IPI
    Hsp70 protein binding GO:0030544 IPI
    polyubiquitin modification-dependent protein binding GO:0031593 IBA
    polyubiquitin modification-dependent protein binding GO:0031593 IDA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    identical protein binding GO:0042802 ISO
    ribosome binding GO:0043022 ISO
    ribosome binding GO:0043022 ISS
    receptor ligand activity GO:0048018 IEA
    misfolded protein binding GO:0051787 IBA
    misfolded protein binding GO:0051787 IEA
    misfolded protein binding GO:0051787 ISO
    molecular adaptor activity GO:0060090 ISO
    proteasome binding GO:0070628 IDA
    ubiquitin-specific protease binding GO:1990381 IEA
    ubiquitin-specific protease binding GO:1990381 ISO
Biological Process
    kidney development GO:0001822 IMP
    immune response-activating cell surface receptor signaling pathway GO:0002429 IEA
    immune response-activating cell surface receptor signaling pathway GO:0002429 ISO
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
    chromatin organization GO:0006325 IEA
    ubiquitin-dependent protein catabolic process GO:0006511 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 ISO
    post-translational protein targeting to endoplasmic reticulum membrane GO:0006620 ISO
    apoptotic process GO:0006915 ISO
    apoptotic process GO:0006915 ISS
    apoptotic process GO:0006915 RCA
    synaptonemal complex assembly GO:0007130 IMP
    spermatogenesis GO:0007283 IMP
    brain development GO:0007420 IMP
    proteasomal protein catabolic process GO:0010498 ISO
    proteasomal protein catabolic process GO:0010498 ISS
    internal peptidyl-lysine acetylation GO:0018393 ISO
    internal peptidyl-lysine acetylation GO:0018393 ISS
    natural killer cell activation GO:0030101 IEA
    natural killer cell activation GO:0030101 ISO
    cell differentiation GO:0030154 IEA
    lung development GO:0030324 IMP
    regulation of protein stability GO:0031647 ISO
    negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032435 IMP
    ERAD pathway GO:0036503 IBA
    ERAD pathway GO:0036503 ISO
    ERAD pathway GO:0036503 ISS
    maintenance of unfolded protein GO:0036506 IEA
    maintenance of unfolded protein GO:0036506 ISO
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 ISO
    intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator GO:0042771 ISS
    regulation of apoptotic process GO:0042981 IMP
    regulation of apoptotic process GO:0042981 RCA
    negative regulation of apoptotic process GO:0043066 IGI
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IMP
    negative regulation of proteolysis GO:0045861 IMP
    regulation of embryonic development GO:0045995 IMP
    protein stabilization GO:0050821 IMP
    NK T cell activation GO:0051132 IEA
    endoplasmic reticulum stress-induced pre-emptive quality control GO:0061857 ISO
    endoplasmic reticulum stress-induced pre-emptive quality control GO:0061857 ISS
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 IMP
    tail-anchored membrane protein insertion into ER membrane GO:0071816 ISO
    tail-anchored membrane protein insertion into ER membrane GO:0071816 ISS
    positive regulation of ERAD pathway GO:1904294 IEA
    positive regulation of ERAD pathway GO:1904294 ISO
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    cytosol GO:0005829 NAS
    membrane GO:0016020 IEA
    membrane GO:0016020 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    BAT3 complex GO:0071818 IBA
    BAT3 complex GO:0071818 ISO
    BAT3 complex GO:0071818 ISS
 Experiment description of studies that identified Bag6 in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Bag6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Aifm1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
2 Ubc  
Reconstituted Complex Mus musculus
3 CAP1 10487
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 Atxn1  
Affinity Capture-MS Mus musculus
5 Eed  
Affinity Capture-MS Mus musculus
6 Traf6  
Affinity Capture-Western Mus musculus
7 Havcr2  
Two-hybrid Mus musculus
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
8 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Bag6 is involved
No pathways found





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