Gene description for Ppp2r5a
Gene name protein phosphatase 2, regulatory subunit B', alpha
Gene symbol Ppp2r5a
Other names/aliases PR61alpha
Species Mus musculus
 Database cross references - Ppp2r5a
ExoCarta ExoCarta_226849
Vesiclepedia VP_226849
Entrez Gene 226849
UniProt Q6PD03  
 Ppp2r5a identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ppp2r5a
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 ISO
    protein binding GO:0005515 IPI
    kinase inhibitor activity GO:0019210 IEA
    kinase binding GO:0019900 IEA
    kinase binding GO:0019900 ISO
    protein phosphatase activator activity GO:0072542 IBA
    protein phosphatase activator activity GO:0072542 IEA
Biological Process
    protein dephosphorylation GO:0006470 ISO
    signal transduction GO:0007165 IEA
    negative regulation of lipid kinase activity GO:0090219 ISO
    negative regulation of protein localization to plasma membrane GO:1903077 IEA
    negative regulation of protein localization to plasma membrane GO:1903077 ISO
Subcellular Localization
    protein phosphatase type 2A complex GO:0000159 IBA
    protein phosphatase type 2A complex GO:0000159 IEA
    protein phosphatase type 2A complex GO:0000159 ISO
    chromosome, centromeric region GO:0000775 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    Z disc GO:0030018 IDA
    Z disc GO:0030018 ISO
    M band GO:0031430 IDA
 Experiment description of studies that identified Ppp2r5a in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ppp2r5a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pygm  
Co-fractionation Mus musculus
2 Foxp3  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Ppp2r5a is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal IEA Reactome
Amplification of signal from the kinetochores IEA Reactome
Antiviral mechanism by IFN-stimulated genes IEA Reactome
Beta-catenin phosphorylation cascade IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle Checkpoints IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Costimulation by the CD28 family IEA Reactome
CTLA4 inhibitory signaling IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
Disassembly of the destruction complex and recruitment of AXIN to the membrane IEA Reactome
EML4 and NUDC in mitotic spindle formation IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Interferon Signaling IEA Reactome
Intracellular signaling by second messengers IEA Reactome
M Phase IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
Mitotic Anaphase IEA Reactome
Mitotic Metaphase and Anaphase IEA Reactome
Mitotic Prometaphase IEA Reactome
Mitotic Spindle Checkpoint IEA Reactome
Negative regulation of MAPK pathway IEA Reactome
Negative regulation of the PI3K/AKT network IEA Reactome
PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling IEA Reactome
PIP3 activates AKT signaling IEA Reactome
PKR-mediated signaling IEA Reactome
Platelet homeostasis IEA Reactome
Platelet sensitization by LDL IEA Reactome
RAF activation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
Resolution of Sister Chromatid Cohesion IEA Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate Formins IEA Reactome
Separation of Sister Chromatids IEA Reactome
Signal Transduction IEA Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by WNT IEA Reactome
TCF dependent signaling in response to WNT IEA Reactome





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