Gene description for Zfp35
Gene name zinc finger protein 35
Gene symbol Zfp35
Other names/aliases Zfp-35
Znf271
mszf13
mszf20-1
mszf28
mszf68
Species Mus musculus
 Database cross references - Zfp35
ExoCarta ExoCarta_22694
Vesiclepedia VP_22694
Entrez Gene 22694
UniProt P15620  
 Zfp35 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Zfp35
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IBA
    DNA binding GO:0003677 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    inflammatory response to antigenic stimulus GO:0002437 IMP
    negative regulation of type 2 immune response GO:0002829 IMP
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    spermatogenesis GO:0007283 IEA
    cell differentiation GO:0030154 IEA
    negative regulation of T-helper 2 cell differentiation GO:0045629 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
 Experiment description of studies that identified Zfp35 in sEVs
1
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Zfp35
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rbak  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Zfp35 is involved
PathwayEvidenceSource
Gene expression (Transcription) IEA Reactome
Generic Transcription Pathway IEA Reactome
RNA Polymerase II Transcription IEA Reactome





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