Gene description for Plac8
Gene name placenta-specific 8
Gene symbol Plac8
Other names/aliases C15
D5Wsu111e
Species Mus musculus
 Database cross references - Plac8
ExoCarta ExoCarta_231507
Vesiclepedia VP_231507
Entrez Gene 231507
UniProt Q9JI48  
 Plac8 identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Plac8
Molecular Function
    chromatin binding GO:0003682 IDA
    protein binding GO:0005515 IPI
Biological Process
    transcription by RNA polymerase II GO:0006366 IDA
    transcription by RNA polymerase II GO:0006366 IGI
    positive regulation of cell population proliferation GO:0008284 IDA
    response to cold GO:0009409 IMP
    negative regulation of multicellular organism growth GO:0040015 IMP
    defense response to bacterium GO:0042742 IMP
    negative regulation of apoptotic process GO:0043066 IDA
    negative regulation of apoptotic process GO:0043066 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 IBA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    brown fat cell differentiation GO:0050873 IMP
    positive regulation of cold-induced thermogenesis GO:0120162 IMP
Subcellular Localization
    nucleus GO:0005634 IC
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified Plac8 in sEVs
1
Experiment ID 303
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
EM
Biophysical techniques
Cd63|Cd81|Cd9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Plac8
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Plac8 is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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