Gene description for Mat2a
Gene name methionine adenosyltransferase II, alpha
Gene symbol Mat2a
Other names/aliases D630045P18Rik
Species Mus musculus
 Database cross references - Mat2a
ExoCarta ExoCarta_232087
Vesiclepedia VP_232087
Entrez Gene 232087
UniProt Q3THS6  
 Mat2a identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mat2a
Molecular Function
    methionine adenosyltransferase activity GO:0004478 IBA
    methionine adenosyltransferase activity GO:0004478 IDA
    methionine adenosyltransferase activity GO:0004478 ISO
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    amino acid binding GO:0016597 ISO
    small molecule binding GO:0036094 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    S-adenosylmethionine biosynthetic process GO:0006556 IBA
    S-adenosylmethionine biosynthetic process GO:0006556 IDA
    S-adenosylmethionine biosynthetic process GO:0006556 IEA
    S-adenosylmethionine biosynthetic process GO:0006556 ISO
    one-carbon metabolic process GO:0006730 IEA
    circadian rhythm GO:0007623 ISO
    response to xenobiotic stimulus GO:0009410 ISO
    response to hormone GO:0009725 ISO
    protein hexamerization GO:0034214 ISO
    protein hexamerization GO:0034214 ISS
    protein heterooligomerization GO:0051291 ISO
    protein heterooligomerization GO:0051291 ISS
    response to cAMP GO:0051591 ISO
    cellular response to methionine GO:0061431 IEA
    cellular response to methionine GO:0061431 ISO
    positive regulation of TORC1 signaling GO:1904263 IEA
    positive regulation of TORC1 signaling GO:1904263 ISO
    cellular response to leukemia inhibitory factor GO:1990830 IEP
Subcellular Localization
    cytosol GO:0005829 IBA
    methionine adenosyltransferase complex GO:0048269 IBA
    methionine adenosyltransferase complex GO:0048269 ISO
    methionine adenosyltransferase complex GO:0048269 ISS
 Experiment description of studies that identified Mat2a in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mat2a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Suv39h1  
Affinity Capture-Western Mus musculus
2 Mettl21e  
Affinity Capture-MS Mus musculus
3 Setdb1  
Affinity Capture-Western Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Atxn1  
Affinity Capture-MS Mus musculus
6 Atg16l1  
Affinity Capture-MS Mus musculus
7 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Mat2a is involved
PathwayEvidenceSource
Biological oxidations IEA Reactome
Metabolism IEA Reactome
Methylation IEA Reactome
Phase II - Conjugation of compounds IEA Reactome





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