Gene description for Prc1
Gene name protein regulator of cytokinesis 1
Gene symbol Prc1
Other names/aliases D7Ertd348e
Species Mus musculus
 Database cross references - Prc1
ExoCarta ExoCarta_233406
Vesiclepedia VP_233406
Entrez Gene 233406
UniProt Q99K43  
 Prc1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prc1
Molecular Function
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IEA
    kinesin binding GO:0019894 ISO
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 ISO
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    cytokinesis GO:0000910 ISO
    microtubule bundle formation GO:0001578 ISO
    positive regulation of cell population proliferation GO:0008284 ISO
    positive regulation of cell population proliferation GO:0008284 ISS
    regulation of cytokinesis GO:0032465 ISO
    regulation of cytokinesis GO:0032465 ISS
    mitotic spindle midzone assembly GO:0051256 IBA
    cell division GO:0051301 IEA
Subcellular Localization
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IEA
    spindle GO:0005819 IBA
    spindle GO:0005819 ISO
    spindle GO:0005819 ISS
    microtubule GO:0005874 IEA
    plasma membrane GO:0005886 ISO
    microtubule cytoskeleton GO:0015630 ISO
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    intercellular bridge GO:0045171 ISO
    contractile ring GO:0070938 ISO
    contractile ring GO:0070938 ISS
    mitotic spindle midzone GO:1990023 IBA
 Experiment description of studies that identified Prc1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prc1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Zbtb7b  
Affinity Capture-MS Mus musculus
2 Atg16l1  
Affinity Capture-MS Mus musculus
3 Fzr1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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