Gene description for Aars
Gene name alanyl-tRNA synthetase
Gene symbol Aars
Other names/aliases AI316495
C76919
sti
Species Mus musculus
 Database cross references - Aars
ExoCarta ExoCarta_234734
Vesiclepedia VP_234734
Entrez Gene 234734
UniProt Q8BGQ7  
 Aars identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Aars
Molecular Function
    tRNA binding GO:0000049 IEA
    tRNA binding GO:0000049 ISO
    aminoacyl-tRNA editing activity GO:0002161 IBA
    aminoacyl-tRNA editing activity GO:0002161 IDA
    aminoacyl-tRNA editing activity GO:0002161 ISO
    Ser-tRNA(Ala) hydrolase activity GO:0002196 IDA
    Ser-tRNA(Ala) hydrolase activity GO:0002196 IMP
    alanine-tRNA ligase activity GO:0004813 IBA
    alanine-tRNA ligase activity GO:0004813 IDA
    alanine-tRNA ligase activity GO:0004813 ISO
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    zinc ion binding GO:0008270 IEA
    amino acid binding GO:0016597 IEA
    amino acid binding GO:0016597 ISO
    peptide lactyltransferase (ATP-dependent) activity GO:0141207 ISS
Biological Process
    tRNA modification GO:0006400 IDA
    tRNA modification GO:0006400 IMP
    tRNA modification GO:0006400 IMP
    alanyl-tRNA aminoacylation GO:0006419 IBA
    alanyl-tRNA aminoacylation GO:0006419 IDA
    alanyl-tRNA aminoacylation GO:0006419 IMP
    alanyl-tRNA aminoacylation GO:0006419 ISO
    cerebellar Purkinje cell layer development GO:0021680 IMP
    positive regulation of hippo signaling GO:0035332 ISS
    negative regulation of neuron apoptotic process GO:0043524 IMP
    neuromuscular process controlling balance GO:0050885 IMP
    neuromuscular process GO:0050905 IMP
    neuron apoptotic process GO:0051402 IMP
    aminoacyl-tRNA metabolism involved in translational fidelity GO:0106074 IEA
    regulation of cytoplasmic translational fidelity GO:0140018 IMP
    negative regulation of signal transduction by p53 class mediator GO:1901797 ISS
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISS
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Aars in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
EM
Biophysical techniques
CD81|FLOT1
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Aars
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Tpd52  
Co-fractionation Mus musculus
2 Eed  
Affinity Capture-MS Mus musculus
3 Sae1 56459
Co-fractionation Mus musculus
4 Tfe3  
Affinity Capture-MS Mus musculus
5 Tpd52l2  
Co-fractionation Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
7 VASP 7408
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Aars is involved
No pathways found





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