Gene description for Phgdh |
Gene name |
3-phosphoglycerate dehydrogenase |
Gene symbol |
Phgdh |
Other names/aliases |
3-PGDH 3PGDH 4930479N23 A10 PGAD PGD PGDH SERA |
Species |
Mus musculus |
Database cross references - Phgdh |
ExoCarta |
ExoCarta_236539 |
Entrez Gene |
236539 |
UniProt |
Q61753
|
Phgdh identified in exosomes derived from the following tissue/cell type |
Brain cancer cells
|
19109410
|
Fibroblasts
|
23260141
|
Macrophages
|
23658846
|
Macrophages
|
23658846
|
Macrophages
|
23658846
|
Neural stem cells
|
25242146
|
Gene ontology annotations for Phgdh |
|
Experiment description of studies that identified Phgdh in exosomes |
1 |
Experiment ID |
26 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
Alix|GAPDH|HSP70
|
EV Cytosolic markers |
✔
CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19109410
|
Organism |
Mus musculus |
Experiment description |
Proteomic and immunologic analyses of brain tumor exosomes. |
Authors |
Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD |
Journal name |
FASEB
|
Publication year |
2008 |
Sample |
Brain cancer cells |
Sample name |
SMA560vIII |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.13-1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [MALDI TOF/TOF] Western blotting |
|
|
2 |
Experiment ID |
210 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD81|FLOT1
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23260141
|
Organism |
Mus musculus |
Experiment description |
Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration. |
Authors |
Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL. |
Journal name |
Cell
|
Publication year |
2012 |
Sample |
Fibroblasts |
Sample name |
Normal-Fibroblasts (L cells) |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
3 |
Experiment ID |
214 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23658846
|
Organism |
Mus musculus |
Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
Authors |
Hassani K, Olivier M. |
Journal name |
PLoS Negl Trop Dis
|
Publication year |
2013 |
Sample |
Macrophages |
Sample name |
Leishmania-infected-Macrophage (J774A.1) |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
4 |
Experiment ID |
215 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23658846
|
Organism |
Mus musculus |
Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
Authors |
Hassani K, Olivier M. |
Journal name |
PLoS Negl Trop Dis
|
Publication year |
2013 |
Sample |
Macrophages |
Sample name |
LPS-treated-Macrophage (J774A.1) |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
5 |
Experiment ID |
216 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23658846
|
Organism |
Mus musculus |
Experiment description |
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis. |
Authors |
Hassani K, Olivier M. |
Journal name |
PLoS Negl Trop Dis
|
Publication year |
2013 |
Sample |
Macrophages |
Sample name |
Normal-Macrophage (J774A.1) |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
6 |
Experiment ID |
264 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63|CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25242146
|
Organism |
Mus musculus |
Experiment description |
Extracellular Vesicles from Neural Stem Cells Transfer IFN-γ via Ifngr1 to Activate Stat1 Signaling in Target Cells |
Authors |
Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino |
Journal name |
Molecular Cell
|
Publication year |
2014 |
Sample |
Neural stem cells |
Sample name |
NPCs - Th2 treated |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.20 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Mass spectrometry |
|
|
Protein-protein interactions for Phgdh |
|
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
No interactions are found.
|
|
Pathways in which Phgdh is involved |
|
|
|