Gene ontology annotations for Phgdh
Experiment description of studies that identified Phgdh in sEVs
1
Experiment ID
26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19109410
Organism
Mus musculus
Experiment description
Proteomic and immunologic analyses of brain tumor exosomes.
Authors
"Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name
FASEB
Publication year
2008
Sample
Brain cancer cells
Sample name
SMA560vIII
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.13-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF/TOF] Western blotting
2
Experiment ID
907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
37309723
Organism
Mus musculus
Experiment description
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name
Proteomics
Publication year
2023
Sample
Colon cancer cells
Sample name
C26
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
3
Experiment ID
210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23260141
Organism
Mus musculus
Experiment description
Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors
"Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name
Cell
Publication year
2012
Sample
Fibroblasts
Sample name
Normal-Fibroblasts (L cells)
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
4
Experiment ID
214
MISEV standards
✔
EM
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23658846
Organism
Mus musculus
Experiment description
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors
"Hassani K, Olivier M."
Journal name
PLoS Negl Trop Dis
Publication year
2013
Sample
Macrophages
Sample name
Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
5
Experiment ID
215
MISEV standards
✔
EM
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23658846
Organism
Mus musculus
Experiment description
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors
"Hassani K, Olivier M."
Journal name
PLoS Negl Trop Dis
Publication year
2013
Sample
Macrophages
Sample name
LPS-treated-Macrophage (J774A.1)
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
6
Experiment ID
216
MISEV standards
✔
EM
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23658846
Organism
Mus musculus
Experiment description
Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors
"Hassani K, Olivier M."
Journal name
PLoS Negl Trop Dis
Publication year
2013
Sample
Macrophages
Sample name
Normal-Macrophage (J774A.1)
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Filtration Protease inhibitors Sucrose density gradient Filtration Ultracentrifugation
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
7
Experiment ID
264
MISEV standards
✘
Biophysical techniques
✔
CD63|CD9
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25242146
Organism
Mus musculus
Experiment description
Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors
"Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name
Molecular Cell
Publication year
2014
Sample
Neural stem cells
Sample name
NPCs - Th2 treated
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.20 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
8
Experiment ID
908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
37309723
Organism
Mus musculus
Experiment description
Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors
"Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name
Proteomics
Publication year
2023
Sample
T-cell lymphoma cells
Sample name
EL4
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
Protein-protein interactions for Phgdh
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
Kctd13
Affinity Capture-MS
Mus musculus
2
Mettl21e
Affinity Capture-MS
Mus musculus
3
Ubc
Reconstituted Complex
Mus musculus
4
Tmem173
Proximity Label-MS
Mus musculus
5
Eed
Affinity Capture-MS
Mus musculus
6
Mapt
Affinity Capture-MS
Mus musculus
7
Atg16l1
Affinity Capture-MS
Mus musculus
8
Rpap3
Co-fractionation
Mus musculus
9
Dok1
Affinity Capture-MS
Mus musculus
10
Fancd2
Affinity Capture-MS
Mus musculus
11
Kcnma1
Affinity Capture-MS
Mus musculus
View the network
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Pathways in which Phgdh is involved