Gene description for Apob
Gene name apolipoprotein B
Gene symbol Apob
Other names/aliases AI315052
Apo B-100
apob-100
apob-48
Species Mus musculus
 Database cross references - Apob
ExoCarta ExoCarta_238055
Vesiclepedia VP_238055
Entrez Gene 238055
UniProt E9Q414  
 Apob identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Apob
Molecular Function
    phospholipid binding GO:0005543 IEA
    phospholipid binding GO:0005543 ISO
    heparin binding GO:0008201 IEA
    heparin binding GO:0008201 ISO
    lipase binding GO:0035473 IEA
    lipase binding GO:0035473 ISO
    receptor ligand activity GO:0048018 IEA
    low-density lipoprotein particle receptor binding GO:0050750 IBA
    low-density lipoprotein particle receptor binding GO:0050750 IEA
    low-density lipoprotein particle receptor binding GO:0050750 ISO
    cholesterol transfer activity GO:0120020 IBA
    cholesterol transfer activity GO:0120020 IEA
    cholesterol transfer activity GO:0120020 ISO
Biological Process
    in utero embryonic development GO:0001701 IMP
    lipid metabolic process GO:0006629 IDA
    lipid metabolic process GO:0006629 IGI
    lipid metabolic process GO:0006629 IMP
    triglyceride mobilization GO:0006642 IBA
    triglyceride mobilization GO:0006642 IMP
    signal transduction GO:0007165 IEA
    spermatogenesis GO:0007283 IMP
    nervous system development GO:0007399 IMP
    cholesterol metabolic process GO:0008203 IEA
    cholesterol metabolic process GO:0008203 ISO
    fertilization GO:0009566 IMP
    response to virus GO:0009615 IEA
    response to carbohydrate GO:0009743 ISO
    post-embryonic development GO:0009791 IMP
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IEA
    positive regulation of macrophage derived foam cell differentiation GO:0010744 ISO
    positive regulation of lipid storage GO:0010884 ISO
    positive regulation of cholesterol storage GO:0010886 IEA
    positive regulation of cholesterol storage GO:0010886 ISO
    lipid catabolic process GO:0016042 IMP
    triglyceride catabolic process GO:0019433 IMP
    triglyceride catabolic process GO:0019433 ISO
    cholesterol transport GO:0030301 IBA
    cholesterol transport GO:0030301 IMP
    cholesterol transport GO:0030301 ISO
    flagellated sperm motility GO:0030317 IMP
    cholesterol efflux GO:0033344 IGI
    low-density lipoprotein particle remodeling GO:0034374 IEA
    low-density lipoprotein particle remodeling GO:0034374 ISO
    low-density lipoprotein particle clearance GO:0034383 IEA
    low-density lipoprotein particle clearance GO:0034383 ISO
    lipoprotein metabolic process GO:0042157 IGI
    lipoprotein metabolic process GO:0042157 IMP
    lipoprotein biosynthetic process GO:0042158 IGI
    lipoprotein catabolic process GO:0042159 IGI
    cholesterol homeostasis GO:0042632 IBA
    cholesterol homeostasis GO:0042632 IGI
    cholesterol homeostasis GO:0042632 IMP
    cholesterol homeostasis GO:0042632 ISO
    lipoprotein transport GO:0042953 IBA
    lipoprotein transport GO:0042953 IGI
    lipoprotein transport GO:0042953 IMP
    regulation of cholesterol biosynthetic process GO:0045540 IMP
    artery morphogenesis GO:0048844 IGI
    establishment of localization in cell GO:0051649 IGI
    cellular response to lipoprotein particle stimulus GO:0071402 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 ISO
    endoplasmic reticulum GO:0005783 IDA
    lipid droplet GO:0005811 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    vesicle lumen GO:0031983 ISO
    mature chylomicron GO:0034359 IBA
    mature chylomicron GO:0034359 IEA
    mature chylomicron GO:0034359 ISO
    very-low-density lipoprotein particle GO:0034361 IBA
    very-low-density lipoprotein particle GO:0034361 IEA
    very-low-density lipoprotein particle GO:0034361 ISO
    low-density lipoprotein particle GO:0034362 IBA
    low-density lipoprotein particle GO:0034362 IEA
    low-density lipoprotein particle GO:0034362 ISO
    intermediate-density lipoprotein particle GO:0034363 IEA
    intermediate-density lipoprotein particle GO:0034363 ISO
    high-density lipoprotein particle GO:0034364 ISO
    chylomicron GO:0042627 ISO
    neuronal cell body GO:0043025 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    endoplasmic reticulum exit site GO:0070971 IEA
    endoplasmic reticulum exit site GO:0070971 ISO
 Experiment description of studies that identified Apob in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Apob
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Armc8  
Co-fractionation Mus musculus
2 Ugt2a3  
Co-fractionation Mus musculus
3 Sec61a1  
Co-fractionation Mus musculus
4 Nell1  
Co-fractionation Mus musculus
5 Apoa4  
Co-fractionation Mus musculus
6 Atp2b4  
Co-fractionation Mus musculus
7 Vps13a  
Co-fractionation Mus musculus
8 Ptprf  
Co-fractionation Mus musculus
9 Dbr1  
Co-fractionation Mus musculus
10 Lipc  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Apob is involved
PathwayEvidenceSource
Binding and Uptake of Ligands by Scavenger Receptors IEA Reactome
Cargo recognition for clathrin-mediated endocytosis IEA Reactome
Cell surface interactions at the vascular wall IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Chylomicron assembly IEA Reactome
Chylomicron clearance IEA Reactome
Chylomicron remodeling IEA Reactome
Clathrin-mediated endocytosis IEA Reactome
Heme signaling IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
LDL clearance IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism IEA Reactome
Metabolism of fat-soluble vitamins IEA Reactome
Metabolism of proteins IEA Reactome
Metabolism of vitamins and cofactors IEA Reactome
Plasma lipoprotein assembly IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
Plasma lipoprotein clearance IEA Reactome
Plasma lipoprotein remodeling IEA Reactome
Platelet homeostasis IEA Reactome
Platelet sensitization by LDL IEA Reactome
Post-translational protein modification IEA Reactome
Post-translational protein phosphorylation IEA Reactome
Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) IEA Reactome
Regulation of TLR by endogenous ligand IEA Reactome
Retinoid metabolism and transport IEA Reactome
Scavenging by Class A Receptors IEA Reactome
Scavenging by Class B Receptors IEA Reactome
Scavenging by Class H Receptors IEA Reactome
Sensory Perception IEA Reactome
Toll-like Receptor Cascades IEA Reactome
Transport of small molecules IEA Reactome
Vesicle-mediated transport IEA Reactome
Visual phototransduction IEA Reactome
VLDL assembly IEA Reactome
VLDL clearance IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here