Gene description for Clca1
Gene name chloride channel calcium activated 1
Gene symbol Clca1
Other names/aliases Clca2
Clca3
gob-5
gob5
Species Mus musculus
 Database cross references - Clca1
ExoCarta ExoCarta_23844
Vesiclepedia VP_23844
Entrez Gene 23844
UniProt Q9D7Z6  
 Clca1 identified in sEVs derived from the following tissue/cell type
Faeces 33431899    
Faeces 33431899    
 Gene ontology annotations for Clca1
Molecular Function
    intracellularly calcium-gated chloride channel activity GO:0005229 IBA
    intracellularly calcium-gated chloride channel activity GO:0005229 IEA
    chloride channel activity GO:0005254 ISA
    metallopeptidase activity GO:0008237 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    proteolysis GO:0006508 IEA
    calcium ion transport GO:0006816 IEA
    chloride transport GO:0006821 ISA
    cellular response to hypoxia GO:0071456 IEA
    chloride transmembrane transport GO:1902476 IEA
Subcellular Localization
    extracellular region GO:0005576 IEA
    plasma membrane GO:0005886 IBA
    microvillus GO:0005902 IDA
    membrane GO:0016020 IDA
    secretory granule GO:0030141 IDA
    zymogen granule membrane GO:0042589 IEA
    zymogen granule membrane GO:0042589 ISO
 Experiment description of studies that identified Clca1 in sEVs
1
Experiment ID 303
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
2
Experiment ID 304
MISEV standards
✔
EM
Biophysical techniques
✔
Cd63|Cd81|Cd9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33431899    
Organism Mus musculus
Experiment description High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance
Authors "Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG."
Journal name Nat Commun
Publication year 2021
Sample Faeces
Sample name Intestinal epithelial cells - C57BL/6
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Lipid
miRNA
Methods used in the study Western blotting
Mass spectrometry
Immunofluorescence
miRNA array
HPLC
 Protein-protein interactions for Clca1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Cftr  
Co-fractionation Mus musculus
2 Wfs1  
Co-fractionation Mus musculus
3 Wsb1  
Co-fractionation Mus musculus
4 Tjp1  
Co-fractionation Mus musculus
5 Tiam2  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Clca1 is involved
PathwayEvidenceSource
Ion channel transport IEA Reactome
Stimuli-sensing channels IEA Reactome
Transport of small molecules IEA Reactome





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