Gene description for Grem1
Gene name gremlin 1
Gene symbol Grem1
Other names/aliases Cktsf1b1
Drm
Grem
ld
Species Mus musculus
 Database cross references - Grem1
ExoCarta ExoCarta_23892
Vesiclepedia VP_23892
Entrez Gene 23892
UniProt O70326  
 Grem1 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Grem1
Molecular Function
    receptor agonist activity GO:0048018 IPI
    vascular endothelial growth factor receptor 2 binding GO:0043184 IPI
    transmembrane receptor protein tyrosine kinase activator activity GO:0030297 IDA
    heparan sulfate proteoglycan binding GO:0043395 TAS
    cytokine activity GO:0005125 IEA
Biological Process
    positive regulation of angiogenesis GO:0045766 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    determination of dorsal identity GO:0048263 ISO
    positive regulation of cardiac muscle cell differentiation GO:2000727 IDA
    positive regulation of NF-kappaB import into nucleus GO:0042346 IDA
    regulation of stress-activated MAPK cascade GO:0032872 IDA
    positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation GO:0003257 IDA
    positive regulation of cell proliferation GO:0008284 ISO
    activation of transmembrane receptor protein tyrosine kinase activity GO:0007171 IDA
    regulation of focal adhesion assembly GO:0051893 IDA
    positive regulation of receptor activity GO:2000273 IPI
    ureteric bud formation GO:0060676 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    negative regulation of cell growth GO:0030308 ISO
    negative regulation of bone mineralization GO:0030502 ISO
    negative regulation of branching involved in ureteric bud morphogenesis GO:0090191 IDA
    negative regulation of bone remodeling GO:0046851 ISO
    limb development GO:0060173 ISO
    collagen fibril organization GO:0030199 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    positive regulation of telomerase activity GO:0051973 ISO
    cell-cell signaling GO:0007267 IMP
    mesenchymal to epithelial transition involved in metanephros morphogenesis GO:0003337 IDA
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IDA
    proximal/distal pattern formation GO:0009954 IMP
    regulation of epithelial to mesenchymal transition GO:0010717 ISO
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    positive regulation of peptidyl-tyrosine autophosphorylation GO:1900086 IDA
    branching involved in ureteric bud morphogenesis GO:0001658 IGI
    negative regulation of canonical Wnt signaling pathway GO:0090090 ISO
    embryonic limb morphogenesis GO:0030326 IMP
    cell migration involved in sprouting angiogenesis GO:0002042 IDA
    positive regulation of branching involved in ureteric bud morphogenesis GO:0090190 IDA
    negative regulation of BMP signaling pathway GO:0030514 ISO
    negative regulation of pathway-restricted SMAD protein phosphorylation GO:0060394 ISO
    endothelial cell migration GO:0043542 IDA
    signal transduction by p53 class mediator GO:0072331 IGI
    positive regulation of receptor internalization GO:0002092 IDA
    positive regulation of protein tyrosine kinase activity GO:0061098 IDA
    negative regulation of leukocyte chemotaxis GO:0002689 ISO
    negative regulation of osteoclast proliferation GO:0090291 ISO
    negative regulation of monocyte chemotaxis GO:0090027 ISO
    organ morphogenesis GO:0009887 IMP
    signal transduction GO:0007165 IPI
    negative regulation of osteoblast proliferation GO:0033689 ISO
    negative regulation of bone mineralization involved in bone maturation GO:1900158 ISO
    negative regulation of bone trabecula formation GO:1900155 ISO
    negative regulation of chondrocyte differentiation GO:0032331 ISO
    angiogenesis GO:0001525 IDA
    cell morphogenesis GO:0000902 ISO
Subcellular Localization
    extracellular space GO:0005615 ISO
    extracellular region GO:0005576 IEA
    cell surface GO:0009986 ISO
 Experiment description of studies that identified Grem1 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Grem1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Grem1 is involved
No pathways found





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