Gene description for Kctd12
Gene name potassium channel tetramerisation domain containing 12
Gene symbol Kctd12
Other names/aliases AU046135
AW538430
Pfet1
Pfetin
Species Mus musculus
 Database cross references - Kctd12
ExoCarta ExoCarta_239217
Vesiclepedia VP_239217
Entrez Gene 239217
UniProt Q6WVG3  
 Kctd12 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Kctd12
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 ISO
    G protein-coupled neurotransmitter receptor activity involved in regulation of postsynaptic membrane potential GO:0099579 IDA
    G protein-coupled neurotransmitter receptor activity involved in regulation of postsynaptic membrane potential GO:0099579 IMP
    G protein-coupled neurotransmitter receptor activity involved in regulation of presynaptic membrane potential GO:0150047 IDA
    G protein-coupled neurotransmitter receptor activity involved in regulation of presynaptic membrane potential GO:0150047 IMP
Biological Process
    regulation of G protein-coupled receptor signaling pathway GO:0008277 IBA
    regulation of G protein-coupled receptor signaling pathway GO:0008277 IGI
    protein homooligomerization GO:0051260 IEA
    regulation of postsynaptic membrane potential GO:0060078 IEA
    regulation of presynaptic membrane potential GO:0099505 IEA
Subcellular Localization
    presynaptic membrane GO:0042734 IBA
    presynaptic membrane GO:0042734 IDA
    presynaptic membrane GO:0042734 IDA
    cell projection GO:0042995 IEA
    receptor complex GO:0043235 IBA
    receptor complex GO:0043235 IDA
    postsynaptic membrane GO:0045211 IBA
    postsynaptic membrane GO:0045211 IDA
    postsynaptic membrane GO:0045211 IDA
    presynaptic active zone membrane GO:0048787 IDA
    GABA-ergic synapse GO:0098982 IDA
    GABA-ergic synapse GO:0098982 IMP
 Experiment description of studies that identified Kctd12 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Kctd12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Hcn1  
Affinity Capture-MS Mus musculus
2 Fancd2  
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Kctd12 is involved
No pathways found





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