Gene description for Esyt1
Gene name extended synaptotagmin-like protein 1
Gene symbol Esyt1
Other names/aliases Fam62a
Mbc2
vp115
Species Mus musculus
 Database cross references - Esyt1
ExoCarta ExoCarta_23943
Vesiclepedia VP_23943
Entrez Gene 23943
UniProt Q3U7R1  
 Esyt1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Esyt1
Molecular Function
    calcium ion binding GO:0005509 IBA
    protein binding GO:0005515 IPI
    calcium-dependent phospholipid binding GO:0005544 IBA
    lipid binding GO:0008289 IEA
    phosphatidylethanolamine binding GO:0008429 IBA
    phosphatidylcholine binding GO:0031210 IBA
    phosphatidylinositol binding GO:0035091 IBA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
    phospholipid transfer activity GO:0120014 ISO
    phospholipid transfer activity GO:0120014 ISS
Biological Process
    phospholipid transport GO:0015914 IEA
    endoplasmic reticulum-plasma membrane tethering GO:0061817 IEA
    intermembrane lipid transfer GO:0120009 ISO
    intermembrane lipid transfer GO:0120009 ISS
Subcellular Localization
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified Esyt1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Esyt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fancd2  
Affinity Capture-MS Mus musculus
2 Tmem173  
Proximity Label-MS Mus musculus
3 Nphp4  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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