Gene description for Tlr2
Gene name toll-like receptor 2
Gene symbol Tlr2
Other names/aliases Ly105
Species Mus musculus
 Database cross references - Tlr2
ExoCarta ExoCarta_24088
Entrez Gene 24088
UniProt Q9QUN7  
 Tlr2 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Tlr2
Molecular Function
    signaling pattern recognition receptor activity GO:0008329 ISO
    protein binding GO:0005515 IPI
    peptidoglycan binding GO:0042834 ISO
    lipopeptide binding GO:0071723 ISO
    receptor activity GO:0004872 ISO
    lipoteichoic acid binding GO:0070891 IDA
    triacyl lipopeptide binding GO:0042497 ISO
    diacyl lipopeptide binding GO:0042498 IDA
    transmembrane signaling receptor activity GO:0004888 IEA
    protein heterodimerization activity GO:0046982 ISO
Biological Process
    response to peptidoglycan GO:0032494 IMP
    positive regulation of tumor necrosis factor biosynthetic process GO:0042535 IMP
    leukotriene metabolic process GO:0006691 ISO
    positive regulation of interleukin-6 production GO:0032755 ISO
    detection of triacyl bacterial lipopeptide GO:0042495 ISO
    positive regulation of nitric-oxide synthase biosynthetic process GO:0051770 IDA
    immune system process GO:0002376 IEA
    negative regulation of interleukin-17 production GO:0032700 IMP
    positive regulation of leukocyte migration GO:0002687 IMP
    positive regulation of interleukin-12 production GO:0032735 IDA
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 IGI
    inflammatory response GO:0006954 ISO
    cellular response to peptidoglycan GO:0071224 IMP
    chloramphenicol transport GO:0042892 IMP
    detection of diacyl bacterial lipopeptide GO:0042496 ISO
    response to fatty acid GO:0070542 ISO
    positive regulation of cytokine secretion GO:0050715 IGI
    induction by symbiont of defense-related host nitric oxide production GO:0052063 IMP
    immune response GO:0006955 IEA
    nitric oxide metabolic process GO:0046209 ISO
    response to bacterial lipoprotein GO:0032493 ISO
    response to molecule of bacterial origin GO:0002237 IEA
    positive regulation of Wnt signaling pathway GO:0030177 ISO
    positive regulation of interleukin-18 production GO:0032741 IDA
    negative regulation of interleukin-12 production GO:0032695 IMP
    positive regulation of interleukin-10 production GO:0032733 ISO
    positive regulation of interferon-beta production GO:0032728 IDA
    response to molecule of fungal origin GO:0002238 IMP
    negative regulation of cell proliferation GO:0008285 ISO
    I-kappaB phosphorylation GO:0007252 ISO
    defense response to Gram-positive bacterium GO:0050830 ISO
    positive regulation of chemokine production GO:0032722 ISO
    toll-like receptor 2 signaling pathway GO:0034134 IEA
    cellular response to triacyl bacterial lipopeptide GO:0071727 ISO
    positive regulation of nitric oxide biosynthetic process GO:0045429 IMP
    positive regulation of tumor necrosis factor production GO:0032760 IMP
    regulation of cytokine secretion GO:0050707 IEA
    cell surface pattern recognition receptor signaling pathway GO:0002752 IMP
    positive regulation of macrophage cytokine production GO:0060907 IMP
    cellular response to diacyl bacterial lipopeptide GO:0071726 ISO
    toll-like receptor signaling pathway GO:0002224 ISO
    positive regulation of toll-like receptor signaling pathway GO:0034123 ISO
    positive regulation of NF-kappaB transcription factor activity GO:0051092 ISO
    positive regulation of NF-kappaB import into nucleus GO:0042346 ISO
    positive regulation of interleukin-8 production GO:0032757 ISO
    negative regulation of growth of symbiont in host GO:0044130 IMP
    microglial cell activation GO:0001774 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    regulation of dendritic cell cytokine production GO:0002730 IDA
    positive regulation of inflammatory response GO:0050729 IEA
    signal transduction GO:0007165 IEA
    positive regulation of oligodendrocyte differentiation GO:0048714 ISO
    response to bacterium GO:0009617 IMP
    cellular response to lipoteichoic acid GO:0071223 ISO
    central nervous system myelin formation GO:0032289 ISO
    innate immune response GO:0045087 TAS
    cellular response to bacterial lipopeptide GO:0071221 TAS
    interleukin-10 production GO:0032613 ISO
    cytokine secretion involved in immune response GO:0002374 ISO
Subcellular Localization
    cell body GO:0044297 ISO
    Toll-like receptor 2-Toll-like receptor 6 protein complex GO:0035355 IPI
    cell projection GO:0042995 ISO
    Toll-like receptor 1-Toll-like receptor 2 protein complex GO:0035354 ISO
    cell surface GO:0009986 ISO
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 IEA
    cytoplasmic vesicle GO:0031410 IEA
    plasma membrane GO:0005886 ISO
    external side of plasma membrane GO:0009897 IDA
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified Tlr2 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Tlr2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tlr2 is involved
No pathways found





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