Gene description for Add1
Gene name adducin 1 (alpha)
Gene symbol Add1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Add1
ExoCarta ExoCarta_24170
Entrez Gene 24170
UniProt Q63028  
 Add1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Add1
Molecular Function
    structural molecule activity GO:0005198 ISO
    protein homodimerization activity GO:0042803 ISO
    actin filament binding GO:0051015 ISO
    calmodulin binding GO:0005516 IEA
    poly(A) RNA binding GO:0044822 ISO
    spectrin binding GO:0030507 ISO
    protein heterodimerization activity GO:0046982 ISO
    protein binding GO:0005515 IPI
    transcription factor binding GO:0008134 ISO
    T cell receptor binding GO:0042608 IDA
Biological Process
    positive regulation of angiogenesis GO:0045766 IDA
    cell morphogenesis GO:0000902 ISO
    cellular response to retinoic acid GO:0071300 IDA
    cytoskeleton organization GO:0007010 TAS
    erythrocyte differentiation GO:0030218 ISO
    negative regulation of actin filament polymerization GO:0030837 IMP
    in utero embryonic development GO:0001701 ISO
    barbed-end actin filament capping GO:0051016 ISO
    positive regulation of protein binding GO:0032092 ISO
    multicellular organism growth GO:0035264 ISO
    actin filament bundle assembly GO:0051017 ISO
    positive regulation of endocytosis GO:0045807 IMP
    ion transport GO:0006811 NAS
    homeostasis of number of cells within a tissue GO:0048873 ISO
    hemoglobin metabolic process GO:0020027 ISO
    cell volume homeostasis GO:0006884 ISO
Subcellular Localization
    postsynaptic density GO:0014069 IDA
    cytoskeleton GO:0005856 ISO
    nucleus GO:0005634 ISO
    synapse GO:0045202 IDA
    plasma membrane GO:0005886 ISO
    dendritic spine GO:0043197 IDA
    intermediate filament cytoskeleton GO:0045111 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    F-actin capping protein complex GO:0008290 ISO
    nucleoplasm GO:0005654 ISO
    dendrite GO:0030425 IDA
    membrane GO:0016020 ISO
    focal adhesion GO:0005925 ISO
    cell GO:0005623 ISO
 Experiment description of studies that identified Add1 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Add1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Add1 is involved
PathwayEvidenceSource
Caspase-mediated cleavage of cytoskeletal proteins IEA Reactome
Miscellaneous transport and binding events IEA Reactome





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