Gene description for Add2
Gene name adducin 2 (beta)
Gene symbol Add2
Other names/aliases beta-ADD
Species Rattus norvegicus
 Database cross references - Add2
ExoCarta ExoCarta_24171
Entrez Gene 24171
UniProt Q05764  
 Add2 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Add2
Molecular Function
    structural constituent of cytoskeleton GO:0005200 NAS
    structural molecule activity GO:0005198 ISO
    actin filament binding GO:0051015 ISO
    actin binding GO:0003779 IEA
    protein heterodimerization activity GO:0046982 ISO
    protein homodimerization activity GO:0042803 ISO
    calmodulin binding GO:0005516 IEA
    spectrin binding GO:0030507 ISO
Biological Process
    actin filament bundle assembly GO:0051017 ISO
    protein complex assembly GO:0006461 ISS
    barbed-end actin filament capping GO:0051016 ISO
    ion transport GO:0006811 IMP
    positive regulation of protein binding GO:0032092 ISO
    hemopoiesis GO:0030097 ISO
Subcellular Localization
    cytoplasmic membrane-bounded vesicle GO:0016023 ISS
    membrane GO:0016020 ISO
    cytoskeleton GO:0005856 NAS
    plasma membrane GO:0005886 IEA
    neuronal postsynaptic density GO:0097481 ISO
    F-actin capping protein complex GO:0008290 ISO
 Experiment description of studies that identified Add2 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Add2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Add2 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here