Gene description for Ak2
Gene name adenylate kinase 2
Gene symbol Ak2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ak2
ExoCarta ExoCarta_24184
Entrez Gene 24184
UniProt P29410  
 Ak2 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Ak2
Molecular Function
    adenylate kinase activity GO:0004017 TAS
    nucleobase-containing compound kinase activity GO:0019205 IDA
    ATP binding GO:0005524 IEA
Biological Process
    response to thyroid hormone GO:0097066 IEP
    dATP metabolic process GO:0046060 IDA
    liver development GO:0001889 IEP
    brain development GO:0007420 IEP
    ADP biosynthetic process GO:0006172 IDA
    adenine metabolic process GO:0046083 TAS
    ATP metabolic process GO:0046034 IDA
    AMP metabolic process GO:0046033 IDA
    nucleotide phosphorylation GO:0046939 TAS
    oxidative phosphorylation GO:0006119 IEP
Subcellular Localization
    sperm flagellum GO:0036126 ISO
    cytosol GO:0005829 IDA
    mitochondrial intermembrane space GO:0005758 IDA
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 ISO
    sperm mitochondrial sheath GO:0097226 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Ak2 in exosomes
1
Experiment ID 225
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 95
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ak2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ak2 is involved
PathwayEvidenceSource
Synthesis and interconversion of nucleotide di- and triphosphates IEA Reactome





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