Gene description for Ak2
Gene name adenylate kinase 2
Gene symbol Ak2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ak2
ExoCarta ExoCarta_24184
Vesiclepedia VP_24184
Entrez Gene 24184
UniProt P29410  
 Ak2 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Ak2
Molecular Function
    adenylate kinase activity GO:0004017 IBA
    adenylate kinase activity GO:0004017 IDA
    adenylate kinase activity GO:0004017 IEA
    adenylate kinase activity GO:0004017 ISO
    adenylate kinase activity GO:0004017 TAS
    nucleoside diphosphate kinase activity GO:0004550 IEA
    ATP binding GO:0005524 IEA
    nucleobase-containing compound kinase activity GO:0019205 IDA
    nucleobase-containing compound kinase activity GO:0019205 IEA
Biological Process
    liver development GO:0001889 IEP
    oxidative phosphorylation GO:0006119 IEP
    nucleobase-containing compound metabolic process GO:0006139 IEA
    ADP biosynthetic process GO:0006172 IBA
    ADP biosynthetic process GO:0006172 IDA
    ADP biosynthetic process GO:0006172 IEA
    AMP metabolic process GO:0046033 IDA
    AMP metabolic process GO:0046033 IEA
    ATP metabolic process GO:0046034 IEA
    dATP metabolic process GO:0046060 IDA
    adenine metabolic process GO:0046083 TAS
    nucleoside monophosphate phosphorylation GO:0046940 IEA
    response to thyroid hormone GO:0097066 IEP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 ISO
    mitochondrial intermembrane space GO:0005758 IEA
    membrane GO:0016020 IEA
    sperm flagellum GO:0036126 ISO
    sperm mitochondrial sheath GO:0097226 ISO
 Experiment description of studies that identified Ak2 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ak2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ak2 is involved
PathwayEvidenceSource
Interconversion of nucleotide di- and triphosphates IEA Reactome
Metabolism IEA Reactome
Metabolism of nucleotides IEA Reactome





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