Gene description for Atp1a2
Gene name ATPase, Na+/K+ transporting, alpha 2 polypeptide
Gene symbol Atp1a2
Other names/aliases RATATPA2
Species Rattus norvegicus
 Database cross references - Atp1a2
ExoCarta ExoCarta_24212
Entrez Gene 24212
UniProt P06686  
 Atp1a2 identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Atp1a2
Molecular Function
    protein binding GO:0005515 IPI
    cation-transporting ATPase activity GO:0019829 IMP
    sodium:potassium-exchanging ATPase activity GO:0005391 ISO
    ATP binding GO:0005524 IMP
    metal ion binding GO:0046872 IEA
    chaperone binding GO:0051087 ISO
    steroid hormone binding GO:1990239 ISO
Biological Process
    regulation of muscle contraction GO:0006937 ISO
    regulation of smooth muscle contraction GO:0006940 ISO
    cellular sodium ion homeostasis GO:0006883 ISO
    visual learning GO:0008542 ISO
    locomotion GO:0040011 ISO
    cellular response to steroid hormone stimulus GO:0071383 ISO
    response to nicotine GO:0035094 IDA
    negative regulation of heart contraction GO:0045822 ISO
    regulation of vasoconstriction GO:0019229 ISO
    regulation of the force of heart contraction GO:0002026 ISO
    regulation of striated muscle contraction GO:0006942 ISO
    neurotransmitter uptake GO:0001504 ISO
    relaxation of cardiac muscle GO:0055119 IC
    response to glycoside GO:1903416 ISO
    regulation of respiratory gaseous exchange by neurological system process GO:0002087 ISO
    cellular potassium ion homeostasis GO:0030007 ISO
    cellular response to mechanical stimulus GO:0071260 IEP
    cardiac muscle contraction GO:0060048 IC
    regulation of cardiac muscle contraction by calcium ion signaling GO:0010882 IC
    regulation of blood pressure GO:0008217 ISO
    sodium ion transport GO:0006814 IDA
    potassium ion transport GO:0006813 IDA
    energy coupled proton transmembrane transport, against electrochemical gradient GO:0015988 TAS
    potassium ion import GO:0010107 ISO
    negative regulation of calcium:sodium antiporter activity GO:1903280 IMP
    negative regulation of calcium ion transmembrane transport GO:1903170 IMP
    ATP hydrolysis coupled proton transport GO:0015991 IMP
    regulation of cardiac muscle cell contraction GO:0086004 IMP
    adult locomotory behavior GO:0008344 ISO
    sodium ion export from cell GO:0036376 ISO
    negative regulation of cytosolic calcium ion concentration GO:0051481 ISO
    negative regulation of striated muscle contraction GO:0045988 ISO
    metabolic process GO:0008152 IEA
Subcellular Localization
    plasma membrane GO:0005886 ISO
    dendritic spine GO:0043197 IDA
    T-tubule GO:0030315 ISO
    cytoplasm GO:0005737 ISO
    neuron projection GO:0043005 IDA
    endosome GO:0005768 IDA
    intercalated disc GO:0014704 IDA
    cytosol GO:0005829 ISO
    extracellular vesicle GO:1903561 ISO
    cell GO:0005623 ISO
    synapse GO:0045202 IDA
    vesicle GO:0031982 IEA
    sarcolemma GO:0042383 ISO
    myelin sheath GO:0043209 ISO
    caveola GO:0005901 IDA
    sodium:potassium-exchanging ATPase complex GO:0005890 ISO
 Experiment description of studies that identified Atp1a2 in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Atp1a2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Atp1a2 is involved
PathwayEvidenceSource
Ion transport by P-type ATPases IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here