Gene description for C4a
Gene name complement component 4A (Rodgers blood group)
Gene symbol C4a
Other names/aliases C4
C4-1
C4b
Species Rattus norvegicus
 Database cross references - C4a
ExoCarta ExoCarta_24233
Vesiclepedia VP_24233
Entrez Gene 24233
UniProt P08649  
 C4a identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for C4a
Molecular Function
    complement component C1q complex binding GO:0001849 ISO
    endopeptidase inhibitor activity GO:0004866 IEA
Biological Process
    inflammatory response GO:0006954 IEA
    complement activation GO:0006956 IBA
    complement activation GO:0006956 ISO
    complement activation GO:0006956 ISO
    complement activation GO:0006956 TAS
    complement activation, classical pathway GO:0006958 IEA
    innate immune response GO:0045087 IEA
    positive regulation of apoptotic cell clearance GO:2000427 ISO
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    extracellular space GO:0005615 IEA
    extracellular space GO:0005615 ISO
    axon GO:0030424 IEA
    axon GO:0030424 ISO
    dendrite GO:0030425 IEA
    dendrite GO:0030425 ISO
    neuronal cell body GO:0043025 ISO
    synapse GO:0045202 IEA
    synapse GO:0045202 ISO
 Experiment description of studies that identified C4a in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for C4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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