Gene description for Calm1
Gene name calmodulin 1
Gene symbol Calm1
Other names/aliases CaMI
Species Rattus norvegicus
 Database cross references - Calm1
ExoCarta ExoCarta_24242
Entrez Gene 24242
UniProt P62161  
 Calm1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Calm1
Molecular Function
    protein N-terminus binding GO:0047485 IMP
    adenylate cyclase binding GO:0008179 IDA
    enzyme regulator activity GO:0030234 IMP
    N-terminal myristoylation domain binding GO:0031997 ISO
    protein kinase binding GO:0019901 ISO
    nitric-oxide synthase regulator activity GO:0030235 IDA
    calcium channel regulator activity GO:0005246 NAS
    type 3 metabotropic glutamate receptor binding GO:0031800 IPI
    protein binding GO:0005515 IPI
    protein phosphatase activator activity GO:0072542 ISO
    nitric-oxide synthase binding GO:0050998 IDA
    titin binding GO:0031432 ISO
    ion channel binding GO:0044325 ISO
    calcium ion binding GO:0005509 ISO
    protein domain specific binding GO:0019904 ISO
    phosphatidylinositol 3-kinase binding GO:0043548 IPI
    thioesterase binding GO:0031996 ISO
    calcium-dependent protein binding GO:0048306 IPI
    phospholipase binding GO:0043274 ISO
Biological Process
    positive regulation of DNA binding GO:0043388 ISO
    substantia nigra development GO:0021762 ISO
    positive regulation of cyclic-nucleotide phosphodiesterase activity GO:0051343 ISO
    calcium-mediated signaling GO:0019722 IMP
    regulation of high voltage-gated calcium channel activity GO:1901841 IMP
    response to amphetamine GO:0001975 IEP
    G2/M transition of mitotic cell cycle GO:0000086 ISO
    response to calcium ion GO:0051592 ISO
    regulation of ryanodine-sensitive calcium-release channel activity GO:0060314 IDA
    regulation of cytokinesis GO:0032465 ISO
    positive regulation of cyclic nucleotide metabolic process GO:0030801 ISO
    regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0010880 ISO
    activation of adenylate cyclase activity GO:0007190 IDA
    positive regulation of protein dephosphorylation GO:0035307 ISO
    regulation of heart rate GO:0002027 ISO
    positive regulation of phosphoprotein phosphatase activity GO:0032516 ISO
    regulation of store-operated calcium channel activity GO:1901339 IC
    regulation of cardiac muscle contraction GO:0055117 ISO
    detection of calcium ion GO:0005513 ISO
    positive regulation of nitric-oxide synthase activity GO:0051000 IDA
    positive regulation of ryanodine-sensitive calcium-release channel activity GO:0060316 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    centrosome GO:0005813 ISO
    sarcomere GO:0030017 ISO
    cytoplasm GO:0005737 IDA
    spindle pole GO:0000922 ISO
    calcium channel complex GO:0034704 ISO
    neuron projection GO:0043005 IDA
    growth cone GO:0030426 IDA
    plasma membrane GO:0005886 IDA
    spindle microtubule GO:0005876 ISO
    nucleus GO:0005634 ISO
    cytosol GO:0005829 TAS
    vesicle GO:0031982 ISO
 Experiment description of studies that identified Calm1 in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 99
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 100
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Calm1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Calm1 is involved
PathwayEvidenceSource
Activation of Ca-permeable Kainate Receptor IEA Reactome
Activation of CaMK IV IEA Reactome
Antigen activates B Cell Receptor (BCR) leading to generation of second messengers IEA Reactome
Ca2+ pathway IEA Reactome
Calmodulin induced events IEA Reactome
Calmodulin induced events IEA Reactome
CaM pathway IEA Reactome
CaM pathway IEA Reactome
Cam-PDE 1 activation IEA Reactome
Cam-PDE 1 activation IEA Reactome
CaMK IV-mediated phosphorylation of CREB IEA Reactome
CaMK IV-mediated phosphorylation of CREB IEA Reactome
CLEC7A (Dectin-1) induces NFAT activation IEA Reactome
CREB phosphorylation through the activation of CaMKII IEA Reactome
CREB phosphorylation through the activation of CaMKK IEA Reactome
DARPP-32 events IEA Reactome
eNOS activation IEA Reactome
FCERI mediated Ca+2 mobilization IEA Reactome
Glycogen breakdown (glycogenolysis) IEA Reactome
Inactivation, recovery and regulation of the phototransduction cascade IEA Reactome
Platelet degranulation IEA Reactome
Ras activation uopn Ca2+ infux through NMDA receptor IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
RHO GTPases activate PAKs IEA Reactome
Smooth Muscle Contraction IEA Reactome
Synthesis of IP3 and IP4 in the cytosol IEA Reactome
Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation IEA Reactome
Translocation of GLUT4 to the plasma membrane IEA Reactome
VEGFR2 mediated cell proliferation IEA Reactome
VEGFR2 mediated vascular permeability IEA Reactome





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