Gene description for Calm3
Gene name calmodulin 3
Gene symbol Calm3
Other names/aliases RNCAMIII
Species Rattus norvegicus
 Database cross references - Calm3
ExoCarta ExoCarta_24244
Entrez Gene 24244
UniProt P62161  
 Calm3 identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Calm3
Molecular Function
    nitric-oxide synthase binding GO:0050998 IDA
    protein kinase binding GO:0019901 IEA
    thioesterase binding GO:0031996 IEA
    ion channel binding GO:0044325 IDA
    adenylate cyclase binding GO:0008179 IDA
    titin binding GO:0031432 IEA
    phospholipase binding GO:0043274 IEA
    protein binding GO:0005515 IPI
    enzyme regulator activity GO:0030234 IMP
    calcium ion binding GO:0005509 IDA
    nitric-oxide synthase regulator activity GO:0030235 IDA
    protein phosphatase activator activity GO:0072542 IEA
    protein N-terminus binding GO:0047485 IMP
    N-terminal myristoylation domain binding GO:0031997 IEA
Biological Process
    response to amphetamine GO:0001975 IEP
    regulation of high voltage-gated calcium channel activity GO:1901841 IMP
    regulation of heart rate GO:0002027 IEA
    G2/M transition of mitotic cell cycle GO:0000086 ISO
    positive regulation of nitric-oxide synthase activity GO:0051000 IDA
    detection of calcium ion GO:0005513 IEA
    calcium-mediated signaling GO:0019722 IMP
    regulation of ryanodine-sensitive calcium-release channel activity GO:0060314 IDA
    positive regulation of cyclic-nucleotide phosphodiesterase activity GO:0051343 IEA
    regulation of cardiac muscle contraction GO:0055117 IEA
    regulation of store-operated calcium channel activity GO:1901339 IC
    regulation of cytokinesis GO:0032465 IEA
    substantia nigra development GO:0021762 IEA
    positive regulation of protein dephosphorylation GO:0035307 IEA
    positive regulation of ryanodine-sensitive calcium-release channel activity GO:0060316 IEA
    activation of adenylate cyclase activity GO:0007190 IDA
    regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0010880 IEA
    positive regulation of phosphoprotein phosphatase activity GO:0032516 IEA
Subcellular Localization
    neuron projection GO:0043005 IDA
    growth cone GO:0030426 IDA
    calcium channel complex GO:0034704 IEA
    centrosome GO:0005813 IEA
    plasma membrane GO:0005886 IDA
    spindle microtubule GO:0005876 IEA
    extracellular exosome GO:0070062 IEA
    cytosol GO:0005829 TAS
    sarcomere GO:0030017 IEA
    spindle pole GO:0000922 IEA
    cytoplasm GO:0005737 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified Calm3 in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 99
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 100
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Calm3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Calm3 is involved
PathwayEvidenceSource
Activation of Ca-permeable Kainate Receptor IEA Reactome
Activation of CaMK IV IEA Reactome
Antigen activates B Cell Receptor (BCR) leading to generation of second messengers IEA Reactome
Ca2+ pathway IEA Reactome
Calmodulin induced events IEA Reactome
Calmodulin induced events IEA Reactome
CaM pathway IEA Reactome
CaM pathway IEA Reactome
Cam-PDE 1 activation IEA Reactome
Cam-PDE 1 activation IEA Reactome
CaMK IV-mediated phosphorylation of CREB IEA Reactome
CaMK IV-mediated phosphorylation of CREB IEA Reactome
CLEC7A (Dectin-1) induces NFAT activation IEA Reactome
CREB phosphorylation through the activation of CaMKII IEA Reactome
CREB phosphorylation through the activation of CaMKK IEA Reactome
DARPP-32 events IEA Reactome
eNOS activation IEA Reactome
FCERI mediated Ca+2 mobilization IEA Reactome
Glycogen breakdown (glycogenolysis) IEA Reactome
Inactivation, recovery and regulation of the phototransduction cascade IEA Reactome
Platelet degranulation IEA Reactome
Ras activation uopn Ca2+ infux through NMDA receptor IEA Reactome
RHO GTPases activate IQGAPs IEA Reactome
RHO GTPases activate PAKs IEA Reactome
Smooth Muscle Contraction IEA Reactome
Synthesis of IP3 and IP4 in the cytosol IEA Reactome
Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation IEA Reactome
Translocation of GLUT4 to the plasma membrane IEA Reactome
VEGFR2 mediated cell proliferation IEA Reactome
VEGFR2 mediated vascular permeability IEA Reactome





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