Gene description for Comt
Gene name catechol-O-methyltransferase
Gene symbol Comt
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Comt
ExoCarta ExoCarta_24267
Entrez Gene 24267
UniProt P22734  
 Comt identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Comt
Molecular Function
    catechol O-methyltransferase activity GO:0016206 ISO
    magnesium ion binding GO:0000287 IEA
Biological Process
    methylation GO:0032259 ISO
    response to organic cyclic compound GO:0014070 IEP
    catechol-containing compound metabolic process GO:0009712 ISO
    female pregnancy GO:0007565 IMP
    regulation of sensory perception of pain GO:0051930 IMP
    negative regulation of renal sodium excretion GO:0035814 IMP
    estrogen metabolic process GO:0008210 IMP
    neurotransmitter catabolic process GO:0042135 IEA
    developmental process GO:0032502 IEP
    S-adenosylhomocysteine metabolic process GO:0046498 TAS
    response to pain GO:0048265 IMP
    multicellular organismal reproductive process GO:0048609 IMP
    response to lipopolysaccharide GO:0032496 IEP
    dopamine metabolic process GO:0042417 ISO
    response to drug GO:0042493 IEP
    positive regulation of homocysteine metabolic process GO:0050668 IMP
    cellular response to phosphate starvation GO:0016036 ISO
    negative regulation of smooth muscle cell proliferation GO:0048662 IMP
    short-term memory GO:0007614 IMP
    learning GO:0007612 IMP
    dopamine catabolic process GO:0042420 ISO
    S-adenosylmethionine metabolic process GO:0046500 TAS
    negative regulation of dopamine metabolic process GO:0045963 IMP
Subcellular Localization
    cytosol GO:0005829 IDA
    membrane GO:0016020 ISO
    dendritic spine GO:0043197 IDA
    plasma membrane GO:0005886 ISO
    integral component of membrane GO:0016021 IEA
    postsynaptic membrane GO:0045211 IDA
    extracellular exosome GO:0070062 ISO
    dendrite GO:0030425 IDA
    cell body GO:0044297 IDA
    axon GO:0030424 IDA
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Comt in exosomes
1
Experiment ID 35
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD81|CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 98
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Comt
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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