Gene description for Dhfr
Gene name dihydrofolate reductase
Gene symbol Dhfr
Other names/aliases Dhfr1
Species Rattus norvegicus
 Database cross references - Dhfr
ExoCarta ExoCarta_24312
Vesiclepedia VP_24312
Entrez Gene 24312
UniProt Q920D2  
 Dhfr identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Dhfr
Molecular Function
    mRNA regulatory element binding translation repressor activity GO:0000900 ISO
    mRNA binding GO:0003729 ISO
    mRNA binding GO:0003729 ISS
    dihydrofolate reductase activity GO:0004146 IBA
    dihydrofolate reductase activity GO:0004146 IDA
    dihydrofolate reductase activity GO:0004146 IMP
    dihydrofolate reductase activity GO:0004146 ISO
    dihydrofolate reductase activity GO:0004146 ISS
    folic acid binding GO:0005542 ISO
    NADP binding GO:0050661 IBA
    NADP binding GO:0050661 IDA
    dihydrofolic acid binding GO:0051871 IDA
    NADPH binding GO:0070402 ISO
    sequence-specific mRNA binding GO:1990825 ISO
Biological Process
    tetrahydrobiopterin biosynthetic process GO:0006729 ISO
    negative regulation of translation GO:0017148 ISO
    axon regeneration GO:0031103 IMP
    response to methotrexate GO:0031427 IEA
    response to nicotine GO:0035094 IEP
    tetrahydrofolate interconversion GO:0035999 ISO
    dihydrofolate metabolic process GO:0046452 IBA
    dihydrofolate metabolic process GO:0046452 IDA
    dihydrofolate metabolic process GO:0046452 ISO
    tetrahydrofolate metabolic process GO:0046653 ISO
    tetrahydrofolate metabolic process GO:0046653 ISS
    tetrahydrofolate biosynthetic process GO:0046654 IBA
    tetrahydrofolate biosynthetic process GO:0046654 IDA
    tetrahydrofolate biosynthetic process GO:0046654 IEA
    tetrahydrofolate biosynthetic process GO:0046654 ISO
    folic acid metabolic process GO:0046655 IBA
    folic acid metabolic process GO:0046655 IDA
    positive regulation of nitric-oxide synthase activity GO:0051000 ISO
    regulation of removal of superoxide radicals GO:2000121 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Dhfr in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Dhfr
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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