Gene description for G6pd
Gene name glucose-6-phosphate dehydrogenase
Gene symbol G6pd
Other names/aliases G6pdx
Species Rattus norvegicus
 Database cross references - G6pd
ExoCarta ExoCarta_24377
Vesiclepedia VP_24377
Entrez Gene 24377
UniProt P05370  
 G6pd identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for G6pd
Molecular Function
    glucose-6-phosphate dehydrogenase activity GO:0004345 IBA
    glucose-6-phosphate dehydrogenase activity GO:0004345 IDA
    glucose-6-phosphate dehydrogenase activity GO:0004345 IMP
    glucose-6-phosphate dehydrogenase activity GO:0004345 ISO
    D-glucose binding GO:0005536 IDA
    D-glucose binding GO:0005536 IMP
    D-glucose binding GO:0005536 ISO
    carbohydrate binding GO:0030246 IDA
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    NADP binding GO:0050661 IDA
    NADP binding GO:0050661 IMP
    NADP binding GO:0050661 ISO
Biological Process
    angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure GO:0001998 ISO
    angiotensin-mediated vasodilation involved in regulation of systemic arterial blood pressure GO:0002033 ISO
    glucose metabolic process GO:0006006 IBA
    pentose-phosphate shunt GO:0006098 IDA
    pentose-phosphate shunt GO:0006098 ISO
    cholesterol biosynthetic process GO:0006695 ISO
    NADP metabolic process GO:0006739 ISO
    NADP metabolic process GO:0006739 ISS
    NADPH regeneration GO:0006740 ISO
    NADP biosynthetic process GO:0006741 ISO
    glutathione metabolic process GO:0006749 ISO
    glutathione metabolic process GO:0006749 ISO
    response to oxidative stress GO:0006979 ISO
    pentose-phosphate shunt, oxidative branch GO:0009051 IBA
    pentose-phosphate shunt, oxidative branch GO:0009051 IMP
    pentose-phosphate shunt, oxidative branch GO:0009051 ISO
    response to iron(III) ion GO:0010041 IEP
    negative regulation of protein glutathionylation GO:0010734 ISO
    response to organic cyclic compound GO:0014070 IEP
    pentose biosynthetic process GO:0019322 ISO
    pentose biosynthetic process GO:0019322 ISO
    substantia nigra development GO:0021762 ISO
    response to food GO:0032094 IEP
    cellular response to oxidative stress GO:0034599 ISO
    regulation of multicellular organism growth GO:0040014 ISO
    erythrocyte maturation GO:0043249 ISO
    regulation of neuron apoptotic process GO:0043523 IDA
    response to ethanol GO:0045471 IDA
    ribose phosphate biosynthetic process GO:0046390 ISO
    erythrocyte development GO:0048821 ISO
    glucose 6-phosphate metabolic process GO:0051156 IDA
    glucose 6-phosphate metabolic process GO:0051156 IMP
    glucose 6-phosphate metabolic process GO:0051156 ISO
    negative regulation of cell growth involved in cardiac muscle cell development GO:0061052 IMP
    positive regulation of calcium ion transmembrane transport via high voltage-gated calcium channel GO:1904879 IMP
    negative regulation of reactive oxygen species metabolic process GO:2000378 IMP
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISO
    centriolar satellite GO:0034451 IEA
    centriolar satellite GO:0034451 ISO
    intracellular membrane-bounded organelle GO:0043231 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
 Experiment description of studies that identified G6pd in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 101
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for G6pd
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fbxo21  
Affinity Capture-MS Rattus norvegicus
2 Tagln  
Affinity Capture-Western Rattus norvegicus
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