Gene description for Gnb1
Gene name guanine nucleotide binding protein (G protein), beta polypeptide 1
Gene symbol Gnb1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gnb1
ExoCarta ExoCarta_24400
Entrez Gene 24400
UniProt P54311  
 Gnb1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Gnb1
Molecular Function
    GTPase activity GO:0003924 ISO
    protein binding GO:0005515 IPI
    signal transducer activity GO:0004871 IEA
    GTPase binding GO:0051020 ISO
    spectrin binding GO:0030507 IDA
    protein complex binding GO:0032403 ISO
    G-protein coupled receptor binding GO:0001664 IMP
    type 1 angiotensin receptor binding GO:0031702 IMP
Biological Process
    positive regulation of cytosolic calcium ion concentration GO:0007204 IMP
    sensory perception of taste GO:0050909 ISO
    G-protein coupled receptor signaling pathway GO:0007186 TAS
    cardiac muscle cell apoptotic process GO:0010659 IDA
    cell proliferation GO:0008283 ISO
    cellular response to hypoxia GO:0071456 IDA
    metabolic process GO:0008152 IEA
    phospholipase C-activating G-protein coupled receptor signaling pathway GO:0007200 ISO
    retina development in camera-type eye GO:0060041 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    dendrite GO:0030425 IDA
    photoreceptor inner segment GO:0001917 IDA
    extracellular vesicle GO:1903561 ISO
    photoreceptor outer segment membrane GO:0042622 IDA
    heterotrimeric G-protein complex GO:0005834 TAS
    lysosomal membrane GO:0005765 ISO
    myelin sheath GO:0043209 ISO
    cell body GO:0044297 IDA
    photoreceptor outer segment GO:0001750 ISO
    cytosol GO:0005829 IMP
 Experiment description of studies that identified Gnb1 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 99
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 101
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gnb1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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