Gene description for Gstp1
Gene name glutathione S-transferase pi 1
Gene symbol Gstp1
Other names/aliases GST-P
Gst3
Gstp
Gstp2
Species Rattus norvegicus
 Database cross references - Gstp1
ExoCarta ExoCarta_24426
Entrez Gene 24426
UniProt P04906  
 Gstp1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Gstp1
Molecular Function
    glutathione binding GO:0043295 IDA
    drug binding GO:0008144 IDA
    kinase regulator activity GO:0019207 ISS
    S-nitrosoglutathione binding GO:0035730 ISO
    JUN kinase binding GO:0008432 ISS
    dinitrosyl-iron complex binding GO:0035731 ISO
    glutathione transferase activity GO:0004364 ISO
Biological Process
    negative regulation of interleukin-1 beta production GO:0032691 ISS
    negative regulation of monocyte chemotactic protein-1 production GO:0071638 ISS
    cellular response to epidermal growth factor stimulus GO:0071364 IEP
    regulation of ERK1 and ERK2 cascade GO:0070372 ISS
    response to amino acid GO:0043200 IEP
    response to mercury ion GO:0046689 IEP
    response to toxic substance GO:0009636 IEP
    negative regulation of nitric-oxide synthase biosynthetic process GO:0051771 ISS
    response to reactive oxygen species GO:0000302 ISS
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 ISO
    cellular response to lipopolysaccharide GO:0071222 ISS
    glutathione metabolic process GO:0006749 ISO
    positive regulation of superoxide anion generation GO:0032930 ISS
    common myeloid progenitor cell proliferation GO:0035726 ISS
    negative regulation of fibroblast proliferation GO:0048147 ISS
    negative regulation of tumor necrosis factor production GO:0032720 ISS
    negative regulation of peroxidase activity GO:2000469 ISO
    negative regulation of protein kinase activity GO:0006469 ISO
    oligodendrocyte development GO:0014003 IEP
    organ regeneration GO:0031100 IEP
    response to nutrient levels GO:0031667 IEP
    negative regulation of stress-activated MAPK cascade GO:0032873 ISS
    cellular response to cell-matrix adhesion GO:0071460 IEP
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISS
    cellular response to glucocorticoid stimulus GO:0071385 IEP
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 ISS
    negative regulation of biosynthetic process GO:0009890 ISS
    negative regulation of leukocyte proliferation GO:0070664 ISS
    negative regulation of MAP kinase activity GO:0043407 ISO
    response to L-ascorbic acid GO:0033591 IEP
    regulation of stress-activated MAPK cascade GO:0032872 ISS
    cellular response to insulin stimulus GO:0032869 IEP
    response to ethanol GO:0045471 IEP
    xenobiotic metabolic process GO:0006805 TAS
    response to estradiol GO:0032355 IEP
    negative regulation of JUN kinase activity GO:0043508 ISS
Subcellular Localization
    cytosol GO:0005829 IDA
    vesicle GO:0031982 ISO
    mitochondrion GO:0005739 ISO
    extracellular space GO:0005615 ISO
    protein complex GO:0043234 ISS
    intracellular GO:0005622 ISS
    plasma membrane GO:0005886 ISO
    nucleus GO:0005634 IDA
    TRAF2-GSTP1 complex GO:0097057 ISO
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Gstp1 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 99
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Gstp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gstp1 is involved
PathwayEvidenceSource
Detoxification of Reactive Oxygen Species IEA Reactome
Glutathione conjugation IEA Reactome





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