Gene description for Hsd3b5
Gene name hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 5
Gene symbol Hsd3b5
Other names/aliases Hsd1
Hsd3b
RATHSDI
Species Rattus norvegicus
 Database cross references - Hsd3b5
ExoCarta ExoCarta_24470
Vesiclepedia VP_24470
Entrez Gene 24470
UniProt P27364  
 Hsd3b5 identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Hsd3b5
Molecular Function
    NAD binding GO:0051287 IDA
    3-beta-hydroxy-delta5-steroid dehydrogenase activity GO:0003854 IDA
    steroid dehydrogenase activity GO:0016229 TAS
    steroid binding GO:0005496 IDA
Biological Process
    progesterone metabolic process GO:0042448 IDA
    regulation of steroid biosynthetic process GO:0050810 IDA
    steroid biosynthetic process GO:0006694 TAS
    oxidation-reduction process GO:0055114 IEA
Subcellular Localization
    intracellular membrane-bounded organelle GO:0043231 IDA
    integral component of membrane GO:0016021 IEA
    endoplasmic reticulum membrane GO:0005789 IEA
    mitochondrial membrane GO:0031966 IEA
 Experiment description of studies that identified Hsd3b5 in exosomes
1
Experiment ID 35
MISEV standards
CEM
EV Biophysical techniques
TSG101|Alix|CD81|CD63
EV Enriched markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Hsd3b5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hsd3b5 is involved
PathwayEvidenceSource
Androgen biosynthesis IEA Reactome
Glucocorticoid biosynthesis IEA Reactome
Mineralocorticoid biosynthesis IEA Reactome





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