Gene description for Ldhb
Gene name lactate dehydrogenase B
Gene symbol Ldhb
Other names/aliases Ldh2
Species Rattus norvegicus
 Database cross references - Ldhb
ExoCarta ExoCarta_24534
Vesiclepedia VP_24534
Entrez Gene 24534
UniProt P42123  
 Ldhb identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ldhb
Molecular Function
    lactate dehydrogenase activity GO:0004457 IDA
    L-lactate dehydrogenase activity GO:0004459 IBA
    L-lactate dehydrogenase activity GO:0004459 IDA
    L-lactate dehydrogenase activity GO:0004459 ISO
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 IPI
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 ISO
    NAD binding GO:0051287 IDA
Biological Process
    lactate metabolic process GO:0006089 IBA
    lactate metabolic process GO:0006089 IDA
    lactate metabolic process GO:0006089 ISO
    pyruvate metabolic process GO:0006090 IBA
    pyruvate metabolic process GO:0006090 ISO
    NAD metabolic process GO:0019674 IDA
Subcellular Localization
    mitochondrion GO:0005739 IBA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial inner membrane GO:0005743 ISS
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
    membrane raft GO:0045121 ISO
    oxidoreductase complex GO:1990204 ISO
 Experiment description of studies that identified Ldhb in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ldhb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mib1  
Affinity Capture-MS Rattus norvegicus
2 Fbxo21  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Ldhb is involved
PathwayEvidenceSource
Aerobic respiration and respiratory electron transport IEA Reactome
Metabolism IEA Reactome
Pyruvate metabolism IEA Reactome





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