Gene description for Lmbrd1
Gene name LMBR1 domain containing 1
Gene symbol Lmbrd1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Lmbrd1
ExoCarta ExoCarta_246046
Vesiclepedia VP_246046
Entrez Gene 246046
UniProt Q6AZ61  
 Lmbrd1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Lmbrd1
Molecular Function
    insulin receptor binding GO:0005158 ISO
    cobalamin binding GO:0031419 IEA
    clathrin heavy chain binding GO:0032050 ISO
    clathrin heavy chain binding GO:0032050 ISS
    AP-2 adaptor complex binding GO:0035612 ISO
    AP-2 adaptor complex binding GO:0035612 ISS
    protein transporter activity GO:0140318 ISO
Biological Process
    gastrulation GO:0007369 ISO
    gastrulation GO:0007369 ISS
    insulin receptor internalization GO:0038016 ISO
    insulin receptor internalization GO:0038016 ISS
    protein localization to lysosome GO:0061462 IBA
    protein localization to lysosome GO:0061462 ISO
    protein localization to lysosome GO:0061462 ISS
    clathrin-dependent endocytosis GO:0072583 ISO
    clathrin-dependent endocytosis GO:0072583 ISS
Subcellular Localization
    lysosome GO:0005764 ISO
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    endoplasmic reticulum membrane GO:0005789 ISO
    endoplasmic reticulum membrane GO:0005789 ISS
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    clathrin-coated vesicle GO:0030136 ISO
    clathrin-coated vesicle GO:0030136 ISS
    intracellular membrane-bounded organelle GO:0043231 ISO
    clathrin-coated endocytic vesicle GO:0045334 ISO
    clathrin-coated endocytic vesicle GO:0045334 ISS
 Experiment description of studies that identified Lmbrd1 in sEVs
1
Experiment ID 96
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 99
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Lmbrd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Lmbrd1 is involved
No pathways found





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