Gene description for Nras
Gene name neuroblastoma ras oncogene
Gene symbol Nras
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Nras
ExoCarta ExoCarta_24605
Vesiclepedia VP_24605
Entrez Gene 24605
UniProt Q04970  
 Nras identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Nras
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    GTPase activity GO:0003924 ISS
    G protein activity GO:0003925 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GDP binding GO:0019003 IBA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    regulation of protein phosphorylation GO:0001932 ISO
    positive regulation of endothelial cell proliferation GO:0001938 ISO
    signal transduction GO:0007165 IEA
    Ras protein signal transduction GO:0007265 IBA
    Ras protein signal transduction GO:0007265 ISO
    Ras protein signal transduction GO:0007265 ISS
    regulation of gene expression GO:0010468 ISO
    positive regulation of type II interferon production GO:0032729 ISO
    defense response to protozoan GO:0042832 ISO
    myoblast differentiation GO:0045445 IEP
    positive regulation of angiogenesis GO:0045766 ISO
    positive regulation of fibroblast proliferation GO:0048146 ISO
    negative regulation of skeletal muscle tissue development GO:0048642 ISO
    T cell receptor signaling pathway GO:0050852 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi apparatus GO:0005794 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 TAS
    membrane GO:0016020 ISO
 Experiment description of studies that identified Nras in sEVs
1
Experiment ID 35
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|CD81|CD63
Enriched markers
HSPA5
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 192
MISEV standards
CEM
Biophysical techniques
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Nras
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rgs12  
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml
 Pathways in which Nras is involved
PathwayEvidenceSource
Activation of RAS in B cells IEA Reactome
Adaptive Immune System IEA Reactome
Axon guidance IEA Reactome
C-type lectin receptors (CLRs) IEA Reactome
CD209 (DC-SIGN) signaling IEA Reactome
Cell surface interactions at the vascular wall IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
DAP12 interactions IEA Reactome
DAP12 signaling IEA Reactome
Developmental Biology IEA Reactome
Downstream signal transduction IEA Reactome
Downstream signaling events of B Cell Receptor (BCR) IEA Reactome
Downstream signaling of activated FGFR1 IEA Reactome
Downstream signaling of activated FGFR2 IEA Reactome
Downstream signaling of activated FGFR3 IEA Reactome
Downstream signaling of activated FGFR4 IEA Reactome
EGFR Transactivation by Gastrin IEA Reactome
ESR-mediated signaling IEA Reactome
Estrogen-stimulated signaling through PRKCZ IEA Reactome
Extra-nuclear estrogen signaling IEA Reactome
Fc epsilon receptor (FCERI) signaling IEA Reactome
FCERI mediated MAPK activation IEA Reactome
FLT3 Signaling IEA Reactome
FRS-mediated FGFR1 signaling IEA Reactome
FRS-mediated FGFR2 signaling IEA Reactome
FRS-mediated FGFR3 signaling IEA Reactome
FRS-mediated FGFR4 signaling IEA Reactome
G alpha (q) signalling events IEA Reactome
Gastrin-CREB signalling pathway via PKC and MAPK IEA Reactome
GPCR downstream signalling IEA Reactome
GRB2 events in EGFR signaling IEA Reactome
GRB2 events in ERBB2 signaling IEA Reactome
Hemostasis IEA Reactome
Immune System IEA Reactome
Innate Immune System IEA Reactome
MAP2K and MAPK activation IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK1/MAPK3 signaling IEA Reactome
MET activates RAS signaling IEA Reactome
NCAM signaling for neurite out-growth IEA Reactome
Negative regulation of MAPK pathway IEA Reactome
Nervous system development IEA Reactome
Neutrophil degranulation IEA Reactome
p38MAPK events IEA Reactome
PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases IEA Reactome
RAF activation IEA Reactome
RAF/MAP kinase cascade IEA Reactome
RAS processing IEA Reactome
Regulation of RAS by GAPs IEA Reactome
SHC-mediated cascade:FGFR1 IEA Reactome
SHC-mediated cascade:FGFR2 IEA Reactome
SHC-mediated cascade:FGFR3 IEA Reactome
SHC-mediated cascade:FGFR4 IEA Reactome
SHC1 events in EGFR signaling IEA Reactome
SHC1 events in ERBB4 signaling IEA Reactome
Signal Transduction IEA Reactome
Signaling by EGFR IEA Reactome
Signaling by ERBB2 IEA Reactome
Signaling by ERBB4 IEA Reactome
Signaling by FGFR IEA Reactome
Signaling by FGFR1 IEA Reactome
Signaling by FGFR2 IEA Reactome
Signaling by FGFR3 IEA Reactome
Signaling by FGFR4 IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by MET IEA Reactome
Signaling by Non-Receptor Tyrosine Kinases IEA Reactome
Signaling by NTRK1 (TRKA) IEA Reactome
Signaling by NTRKs IEA Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by PDGF IEA Reactome
Signaling by PTK6 IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by SCF-KIT IEA Reactome
Signaling by the B Cell Receptor (BCR) IEA Reactome
Signaling by VEGF IEA Reactome
Signalling to ERKs IEA Reactome
Signalling to RAS IEA Reactome
Tie2 Signaling IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome
VEGFR2 mediated cell proliferation IEA Reactome





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