Gene description for Nras
Gene name neuroblastoma ras oncogene
Gene symbol Nras
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Nras
ExoCarta ExoCarta_24605
Entrez Gene 24605
UniProt Q04970  
 Nras identified in exosomes derived from the following tissue/cell type
Hepatocytes 19367702    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Nras
Molecular Function
    GTP binding GO:0005525 IEA
    molecular_function GO:0003674 ND
    protein complex binding GO:0032403 ISO
    protein binding GO:0005515 IPI
Biological Process
    regulation of synaptic transmission, GABAergic GO:0032228 IEA
    negative regulation of skeletal muscle tissue development GO:0048642 ISO
    striated muscle cell differentiation GO:0051146 IEA
    positive regulation of fibroblast proliferation GO:0048146 ISO
    actin cytoskeleton organization GO:0030036 IEA
    biological_process GO:0008150 ND
    regulation of gene expression GO:0010468 ISO
    regulation of long-term neuronal synaptic plasticity GO:0048169 IEA
    epithelial tube branching involved in lung morphogenesis GO:0060441 IEA
    negative regulation of neuron apoptotic process GO:0043524 IEA
    positive regulation of Rac protein signal transduction GO:0035022 IEA
    regulation of protein phosphorylation GO:0001932 ISO
    establishment of protein localization to Golgi GO:0072600 ISO
    negative regulation of cell differentiation GO:0045596 IEA
    visual learning GO:0008542 IEA
    small GTPase mediated signal transduction GO:0007264 IEA
    positive regulation of cell proliferation GO:0008284 IEA
Subcellular Localization
    membrane GO:0016020 ISO
    extracellular exosome GO:0070062 ISO
    Golgi apparatus GO:0005794 ISO
    Golgi membrane GO:0000139 IEA
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified Nras in exosomes
1
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Nras
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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