Gene description for Myo1g
Gene name myosin IG
Gene symbol Myo1g
Other names/aliases E430002D17Rik
Species Mus musculus
 Database cross references - Myo1g
ExoCarta ExoCarta_246177
Vesiclepedia VP_246177
Entrez Gene 246177
UniProt Q5SUA5  
 Myo1g identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Myo1g
Molecular Function
    microfilament motor activity GO:0000146 IBA
    cytoskeletal motor activity GO:0003774 TAS
    actin binding GO:0003779 IEA
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IDA
    actin filament binding GO:0051015 IBA
Biological Process
    T cell mediated immunity GO:0002456 IMP
    exocytosis GO:0006887 IMP
    endocytosis GO:0006897 IBA
    phagocytosis GO:0006909 IMP
    actin filament organization GO:0007015 IBA
    actin filament-based movement GO:0030048 IBA
    cell-substrate adhesion GO:0031589 IMP
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 IMP
    establishment of localization in cell GO:0051649 IMP
    cell gliding GO:0071976 IMP
    T cell meandering migration GO:0120117 IMP
Subcellular Localization
    phagocytic cup GO:0001891 IDA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 ISO
    microvillus GO:0005902 IBA
    microvillus GO:0005902 IDA
    actin cytoskeleton GO:0015629 IBA
    myosin complex GO:0016459 IEA
    lamellipodium GO:0030027 IDA
    filopodium GO:0030175 IDA
    leading edge membrane GO:0031256 IDA
 Experiment description of studies that identified Myo1g in sEVs
1
Experiment ID 907
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
EM
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
EM
Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
HSPA5
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Myo1g
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rgag1  
Co-fractionation Mus musculus
2 Sik1  
Co-fractionation Mus musculus
3 Strbp  
Co-fractionation Mus musculus
4 Tfrc 22042
Co-fractionation Mus musculus
5 Cd247  
Co-fractionation Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
7 MTNR1B  
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
View the network image/svg+xml
 Pathways in which Myo1g is involved
No pathways found





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