Gene description for Tpcn1
Gene name two pore segment channel 1
Gene symbol Tpcn1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Tpcn1
ExoCarta ExoCarta_246215
Vesiclepedia VP_246215
Entrez Gene 246215
UniProt Q9WTN5  
 Tpcn1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tpcn1
Molecular Function
    monoatomic ion channel activity GO:0005216 IEA
    voltage-gated sodium channel activity GO:0005248 IEA
    voltage-gated sodium channel activity GO:0005248 ISO
    voltage-gated sodium channel activity GO:0005248 ISS
    ligand-gated sodium channel activity GO:0015280 IEA
    ligand-gated sodium channel activity GO:0015280 ISO
    ligand-gated sodium channel activity GO:0015280 ISS
    syntaxin binding GO:0019905 IEA
    syntaxin binding GO:0019905 ISO
    voltage-gated channel activity GO:0022832 IEA
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    NAADP-sensitive calcium-release channel activity GO:0072345 IEA
    NAADP-sensitive calcium-release channel activity GO:0072345 ISO
    NAADP-sensitive calcium-release channel activity GO:0072345 ISO
    NAADP-sensitive calcium-release channel activity GO:0072345 ISS
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IEA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 ISO
    intracellularly phosphatidylinositol-3,5-bisphosphate-gated monatomic cation channel activity GO:0097682 IEA
    intracellularly phosphatidylinositol-3,5-bisphosphate-gated monatomic cation channel activity GO:0097682 ISO
    intracellularly phosphatidylinositol-3,5-bisphosphate-gated monatomic cation channel activity GO:0097682 ISS
Biological Process
    biological_process GO:0008150 ND
    positive regulation of autophagy GO:0010508 IEA
    positive regulation of autophagy GO:0010508 ISO
    calcium-mediated signaling GO:0019722 IEA
    monoatomic ion transmembrane transport GO:0034220 IEA
    sodium ion transmembrane transport GO:0035725 IEA
    sodium ion transmembrane transport GO:0035725 ISO
    calcium ion transmembrane transport GO:0070588 IEA
    endocytosis involved in viral entry into host cell GO:0075509 IEA
    endocytosis involved in viral entry into host cell GO:0075509 ISO
Subcellular Localization
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    endosome membrane GO:0010008 IBA
    endosome membrane GO:0010008 ISO
    endosome membrane GO:0010008 ISS
    membrane GO:0016020 ISO
    early endosome membrane GO:0031901 IEA
    early endosome membrane GO:0031901 ISO
    early endosome membrane GO:0031901 ISS
    monoatomic ion channel complex GO:0034702 IEA
    endolysosome GO:0036019 IEA
    endolysosome GO:0036019 ISO
    endolysosome GO:0036019 ISS
    recycling endosome membrane GO:0055038 IEA
    recycling endosome membrane GO:0055038 ISO
    recycling endosome membrane GO:0055038 ISS
 Experiment description of studies that identified Tpcn1 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 99
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 100
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tpcn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tpcn1 is involved
PathwayEvidenceSource
Ion channel transport IEA Reactome
Stimuli-sensing channels IEA Reactome
Transport of small molecules IEA Reactome





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