Gene description for Ppm1a
Gene name protein phosphatase, Mg2+/Mn2+ dependent, 1A
Gene symbol Ppm1a
Other names/aliases Pp2c1
Species Rattus norvegicus
 Database cross references - Ppm1a
ExoCarta ExoCarta_24666
Entrez Gene 24666
UniProt P20650  
 Ppm1a identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Ppm1a
Molecular Function
    protein serine/threonine phosphatase activity GO:0004722 ISO
    phosphoprotein phosphatase activity GO:0004721 ISO
    calmodulin-dependent protein phosphatase activity GO:0033192 ISO
    manganese ion binding GO:0030145 IEA
    signal transducer activity GO:0004871 ISO
    magnesium ion binding GO:0000287 IEA
    protein C-terminus binding GO:0008022 IPI
    R-SMAD binding GO:0070412 ISS
    protein binding GO:0005515 IPI
Biological Process
    dephosphorylation GO:0016311 ISO
    negative regulation of BMP signaling pathway GO:0030514 ISO
    Wnt signaling pathway GO:0016055 ISO
    N-terminal protein myristoylation GO:0006499 ISS
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 ISO
    positive regulation of I-kappaB kinase/NF-kappaB signaling GO:0043123 ISO
    cellular response to transforming growth factor beta stimulus GO:0071560 ISO
    negative regulation of NF-kappaB import into nucleus GO:0042347 ISS
    peptidyl-threonine dephosphorylation GO:0035970 ISO
    positive regulation of transcription, DNA-templated GO:0045893 ISO
    negative regulation of I-kappaB kinase/NF-kappaB signaling GO:0043124 ISS
    positive regulation of protein export from nucleus GO:0046827 ISO
    protein dephosphorylation GO:0006470 ISS
    negative regulation of SMAD protein complex assembly GO:0010991 ISO
    positive regulation of Wnt signaling pathway GO:0030177 ISO
Subcellular Localization
    neuron projection GO:0043005 IDA
    nucleus GO:0005634 ISO
    voltage-gated calcium channel complex GO:0005891 IDA
    membrane GO:0016020 ISS
    cytosol GO:0005829 ISS
 Experiment description of studies that identified Ppm1a in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ppm1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ppm1a is involved
PathwayEvidenceSource
Downregulation of SMAD2/3:SMAD4 transcriptional activity IEA Reactome
Regulation of AMPK activity via LKB1 IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here